The therapeutic effects of antivirus compound on duck hepatitis B viral infection

             Xu  Xiangtian  Wang Zheng

                         (Liver Disease Research Institute of Shandong Medical College, Linyi, China 276002)

                                                        Chen Hongshan  Li Zhuang

(Medical Biotechnology Institute of Chinese Academy of Medical Science)  

Abstract Objective To explore the inhibitory action of antivirus compound (ATVC) on duck hepatitis B virus ( DHBV) by applying ATVC to treat duck hepatitis B viral infection. Methods Intravenous injection of DHBV was given to one-day Beijing ducks in experiment. Seven days later, the ducks received oral administration of ATVC with three different doses of 1.0 g/kg, 2.0 g/kg and 4.0 g/kg, twice a day, for 10 days continuously. The toxicity of ATVC and the effects of ATVC on DHBV-DNA were observed and the results were compared with those got from the use of Lamivudine. Results In the first batch of experiments, the content of DHBV-DNA in duck blood serum in the 4.0g/kg group decreased significantly 10 days after administration of ATVC and three days after drug withdrawal (P<0.05). The pairing statistics showed that the content of DHBV-DNA in duck blood serum decreased significantly in the 5^th day after drug administration and 3^rd of drug withdrawal in the 2.0g/kg group(P<0.01), while with no significant difference in the 1.0g/kg group. In the second batch of experiments, the pairing statistics showed that the content of DHBV-DNA decreased significantly in the 5^th  day of drug administration in the 4.0g/kg group(P<0.05), in the 10^th day of drug administration and three days after drug withdrawal in the 2.0g/kg group(P<0.01), and three days after drug withdrawal in the 1.0g/kg group(P<0.05) respectively. In the third batch of experiments, the pairing statistics showed that the content of DHBV-DNA decreased significantly in the 10^th day of drug administration and three days after drug withdrawal in the 4.0g/kg group(P<0.01), in the 10^th day of drug administration in the 2.0g/kg group(P<0.05), in the 5th and 10^th days of drug administration in the 1.0g/kg group (P<0.05) respectively. In the contrast group of 50mg/kg, Lamivudine was given and the content of DHBV-DNA decreased significantly in the 10^th day of drug administration (P<0.01). Conclusion ATVC can produce inhibitory action in the groups of 2.0-4.0g/kg when ATVC is given twice a day for ten days continuously, but when compared with the contrast group, only the 4.0g/kg group can produce obvious inhibitory action, with no obvious effects in the 1.0g/kg group.

Key words Duck hepatitis B virus( DHBV); DHBV-DNA; Antivirus conmpound; Lamivudine