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Acetic acid (醋酸或乙酸) 溶液的配制
程琳 2024-2-18 12:31
问:如何配制1M的acetic acid溶液? 答:先计算母液的浓度。0.99 (purity)* 1.049 g/mL (density) / 60.05 g/ m ol ( molecular weight ) =0.0173 mol/mL = 13.7 mol/L = 17.3 M 所以,1M acetic acid溶液是将母液稀释17.3倍得到的,即1mL母液溶于16.3 mL dH2O中。
374 次阅读|没有评论
LC-MS
程琳 2023-12-1 05:34
Liquid chromatography-massspectrometry has been used to detect the 3 different change levels. 1. Drug concentration 2. Drug metabolites (metabolomics) 3. Proteinmics (the protein level changes due to drug intake) Sam runs the University of Chicago Core of ...
372 次阅读|没有评论
慢病毒、腺相关病毒、核糖核蛋白作为载体对比
程琳 2023-9-26 23:27
404 次阅读|没有评论
WB control loading antibody
程琳 2023-5-25 21:40
631 次阅读|没有评论
Seahorse notes
程琳 2023-5-17 05:48
Oligomycin: a complex V inhibitor, derive ATP-linked respiration and proton leak respiration. FCCP: ie. proton (H + ), for the maximum capacity ofH + Rotenone: shut down ETC (electron transport chain) function, revealing the non-mitochondrial respiration.non-mutdrome consumptio ...
504 次阅读|没有评论
Immune cell markers
程琳 2023-5-5 03:33
CD3: Universal T cells CD4: helper T cells CD8: cytotoxic T cells CD19: B cells CD21: mature B cells/ follicular dendritic cells CD25: T regulatory cells FoxP3: T regulatory cells (more specific) IFN: interferon cytokines
503 次阅读|没有评论
Cell death markers
程琳 2023-4-9 10:15
Bcl-2 superfamily Pro-apoptotic Bax, Bid, Bok, Bik, Bak, Noxa, Bim, Puma, Bad Anti-apoptotic BCL-2, A1, BCL-XL, BCL-W, BLFL-1, BCL-B, MCL-1 Caspases Initiator: Caspase 2,Caspase 8,Caspase 9,Caspase 10 Effector:Caspase 3,Caspase 6,Casp ...
539 次阅读|没有评论
glutamine vs. glutamate
程琳 2023-3-29 00:07
glutamine vs. glutamate (glutamate =L-Glutamic acid ) 谷氨酰胺vs.谷氨酸 它们是不同的氨基酸 Glutamine (谷氨酰胺)is a conditionally essential amino acid that has various functions of the body. Glutamate (谷氨酸) is a non-essential amino acid whic ...
1150 次阅读|没有评论
how to know if a gene is a transcription factor?
热度 1 程琳 2023-3-25 11:01
To qualify as a transcription factor, a protein must possess two qualities. 1) Ability to bind to DNA and 2) recruit RNA polymerase/alter transcription of a gene. The most common GTFs are TFIIA, TFIIB, TFIID (see also TATA binding protein), TFIIE, TFIIF, and TFIIH . ...
1314 次阅读|1 个评论 热度 1
PCR中DMSO该加多少浓度可以提高反应的特异性?
程琳 2023-3-22 23:13
一般而言,加入终浓度2.5%的DMSO可增加PCR的特异性。KJ建议加终浓度为5%的DMSO,可降解二聚体结构产生的可能。
1539 次阅读|没有评论

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