Gao F . Shen XZ . Jiang F . Wu Y . Han C .
Department of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang, Hebei, China.
来源: Nat Biotechnol ( P 1087-0156 E 1546-1696 ) IF:41.514 H指数:251 年: 2016
PMID: 27136078 [Pubmed] 生物工程与应用微生物,1区

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  Title	DNA-guided genome editing using the Natronobacterium gregoryi Argonaute
  Published in	Nature Biotechnology, May 2016
  DOI	10.1038/nbt.3547
  Pubmed ID	27136078
  Authors	Feng Gao, Xiao Z Shen, Feng Jiang, Yongqiang Wu, Chunyu Han, Gao, Feng, Shen, Xiao Z, Jiang, Feng, Wu, Yongqiang, Han, Chunyu
  Abstract	The RNA-guided endonuclease Cas9 has made genome editing a widely accessible technique. Similar to Cas9, endonucleases from the Argonaute protein family also use oligonucleotides as guides to degrade invasive genomes. Here we report that the Natronobacterium gregoryi Argonaute (NgAgo) is a DNA-guided endonuclease suitable for genome editing in human cells. NgAgo binds 5' phosphorylated single-stranded guide DNA (gDNA) of ～24 nucleotides, efficiently creates site-specific DNA double-strand breaks when loaded with the gDNA. The NgAgo-gDNA system does not require a protospacer-adjacent motif (PAM), as does Cas9, and preliminary characterization suggests a low tolerance to guide-target mismatches and high efficiency in editing (G+C)-rich genomic targets.

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