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最新:研究者成功重复韩氏 NgAgo 方法

已有 10540 次阅读 2016-7-14 08:37 |个人分类:反民科|系统分类:海外观察

更新《研究者称成功重复韩氏 NgAgo 方法

学者成功重复韩氏法. 核酸转染比例达到 70-80%,继续等待测序结果。【Our transfection rates are pretty high, reaching 70 80% and NgAgo reducing plasmid expressed gfp is pretty reproducible.

参见《对韩春雨论文的质疑不应在暗处》。



Debojyoti Chakraborty
Jul 12
Other recipients: haijunt...@gmail.com, crisp...@gmail.com, o.ku...@gmail.com

Hi Haijun,

Our transfection rates are pretty high, reaching 70-80% and NgAgo reducing plasmid expressed gfp is pretty reproducible. We have controls including non Phosphorylated gDNAs which do not give a reduction in GFP so what we see cannot be explained by transfection related issues. We are doing more tests to look at all possibilities and would be able to comment once we are done.

Best
Debo

- show quoted text -
Xiaobing Qing
Jul 12
Other recipients: haijunt...@gmail.com, crisp...@gmail.com, o.ku...@gmail.com
Dear Debo,
Very good news. I'm curious about how you set up the controls.
Did you have a control group with 5'-P-ssDNA but without NgAgo? At least from what you said I couldn't see whether the 5'-P-ssDNA will affect the GFP expression by its own. If I remember it correctly, phosphorylated oligos are toxic to cells.
Waiting for your sequencing results.
Best
Xiaobing

在 2016年7月12日星期二 UTC+2下午5:55:50,Debojyoti Chakraborty写道:
- show quoted text -
Debojyoti Chakraborty
Jul 12
Other recipients: qingxi...@gmail.com, crisp...@gmail.com, o.ku...@gmail.com, haijunt...@gmail.com


Dear Xiaobing,

I would guess that too. So far controls have been scrambled and non Phosphorylated gDNAs. Haven't finished looking for indels or sequencing yet, guess that would be the most telling experiment..

Best
Debo

Dr.Debojyoti Chakraborty

Post Doctoral Researcher [December 2013 – November 2015]

Max Planck Insitute of Molecular Cell Biology and Genetics & Technical University, Dresden,Germany

PhD in Molecular Biology, 2014 [Magna Cum Laude]

Thesis title: Systematic dissection of long noncoding RNAs involved in the regulation of embryonicstem cell pluripotency

German Stem Cell Network Travel Award, Frankfurt, Germany [2015]

German Stem Cell Network Conference Poster Prize, Heidelberg, Germany [2014]

Nucleic Acids Research Poster Prize, Heidelberg, Germany [2014

]Dresden International Graduate School Travel Award, Dresden, Germany [2012]

Future of Science Foundation Scholarship, Keystone Symposia, Utah, USA (2012)




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