Science——科学家开发星型胶质细胞3D钙成像技术

Title and Authors

Abstract

1.Gray matter astrocytes are 3D cells poorly covered by 2D imaging.

2.A 3D imaging and analysis approach to study Ca2+ dynamics in entire astrocytes.

3.3D astrocytic Ca2+ activity in vivo and ex vivo: Comparison with 2D.

4.Astrocytic Ca2+ activity in processes and soma is highly heterogeneous.

5.Endfeet of the same astrocyte are independently active but occasionally show local or global coordination.

6.Hippocampal axons have complex 3D morphological interactions with astrocytes: A functional study with 3D Ca2+ imaging.

7.Astrocytes detect and respond to minimal axonal stimulation in a small portion of their volume.

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