本论文于2017年11月17日在线发表于Journal of Experimental Botany，原文题目如下：

Arabidopsis inositol polyphosphate multikinase delays flowering time through mediating transcriptional activation of FLOWERING LOCUS C

及时开花对植物成功繁殖和种子产量至关重要。已经发现各种各样的调节子通过响应环境和内源信号来控制开花时间。在这些调节子中，FLOWERING LOCUS C（FLC）通过阻断开花整合子基因的表达而充当成花转变的中心抑制子。这里，我们报道，拟南芥肌醇多磷酸酯多激酶（AtIPK2β）通过介导转录调控FLC在染色质的水平来控制开花时间。atipk2β突变体开花较早，AtIPK2β过表达植物表现出晚开花表型。qRT-PCR显示AtIPK2β促进FLC表达。我们进行ChIP-qPCR分析，发现AtIPK2β结合FLC染色质。进一步的分析表明，AtIPK2β与FVE（一种FLC表观遗传沉默所需的关键阻遏物）相互作用。qRT-PCR，ChIP-qPCR和遗传分析表明AtIPK2β通过抑制FVE在FLC上的积累，参与FVE介导的对FLC的转录调控。此外，我们发现AtIPK2β与HDA6相关，HDA6是介导FLC染色质沉默的FVE的相互作用伙伴，并且减弱FLC基因座处的HDA6积累。总之，这些发现表明AtIPK2β通过阻断FLC的染色质沉默来负调控开花时间

Timely flowering is critical for successful reproduction and seed yield in plants. A diverse range of regulators have been found to control flowering time in response to environmental and endogenous signals. Among these regulators, FLOWERING LOCUS C (FLC) acts as a central repressor of floral transition by blocking the expression of flowering integrator genes. Here, we report that Arabidopsis inositol polyphosphate multikinase (AtIPK2β) functions in flowering time control by mediating transcriptional regulation of FLC at the chromatin level. The atipk2β mutant flowers earlier, and AtIPK2β overexpressing plants exhibit late-flowering phenotypes. Quantitative reverse transcription-PCR (qRT-PCR) revealed that AtIPK2β promotes FLC expression. We performed chromatin immunoprecipitation-qPCR (ChIP-qPCR) assays and found that AtIPK2β binds to FLC chromatin. Further analysis showed that AtIPK2β interacts with FVE, a key repressor required for epigenetic silencing of FLC. qRT-PCR, ChIP-qPCR, and genetic analysis demonstrated that AtIPK2β is involved in FVE-mediated transcriptional regulation of FLC by repressing the accumulation of FVE on FLC. Moreover, we found that AtIPK2β associates with HDA6, an interaction partner of FVE mediating FLC chromatin silencing, and attenuates HDA6 accumulation at the FLC locus. Taken together, these findings suggest that AtIPK2β negatively regulates flowering time by blocking chromatin silencing of FLC.

每日一词：

inositol

英[ɪ'nəʊsətəʊl]

美[ɪ'noʊsɪˌtoʊl]

n. 纤维醇，纤维糖，肌糖;

[例句]It is composed mostly of B vitamins, phosphoric acid, choline, linoleic acid and inositol.

它是组成，其中大部分B族维生素，磷酸，胆碱，亚油酸和肌醇。