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磁性纳米颗粒作为基因载体进行遗传修饰的花粉磁转染

已有 2787 次阅读 2017-11-30 14:12 |系统分类:论文交流

本论文于2017年11月27日在线发表于Nature Plants,原文题目如下:

Pollen magnetofection for genetic modification with magnetic nanoparticles as gene carriers


摘要:

遗传修饰对培育具有优良特性的新作物起着至关重要的作用。目前几乎所有的基因修饰方法都需要从组织培养中再生,涉及复杂,长期和艰苦的过程。特别是棉花等许多作物品种难以再生。在这里,我们报告了一种新的转化技术,花粉磁转染,它可以直接产生转基因种子而不需要再生。在这个系统中,载有外源DNA的磁性纳米颗粒在磁场存在下被传递到花粉中。通过磁转染花粉授粉,转化的种子可以成功地产生转基因植物。外源DNA成功整合到基因组中,在后代中有效表达并稳定遗传。我们的系统是无需培养和基因型独立的。另外,它简单,快速,能够进行多基因转化。我们预计花粉磁转染几乎可以转化所有作物,大大促进转基因作物新品种的育种进程。


Abstract:

Genetic modification plays a vital role in breeding new crops with excellent traits. Almost all the current genetic modification methods require regeneration from tissue culture, involving complicated, long and laborious processes. In particular, many crop species such as cotton are difficult to regenerate. Here, we report a novel transformation platform technology, pollen magnetofection, to directly produce transgenic seeds without regeneration. In this system, exogenous DNA loaded with magnetic nanoparticles was delivered into pollen in the presence of a magnetic field. Through pollination with magnetofected pollen, transgenic plants were successfully generated from transformed seeds. Exogenous DNA was successfully integrated into the genome, effectively expressed and stably inherited in the offspring. Our system is culture-free and genotype independent. In addition, it is simple, fast and capable of multi-gene transformation. We envision that pollen magnetofection can transform almost all crops, greatly facilitating breeding processes of new varieties of transgenic crops.


每日一词:

envision

英[ɪnˈvɪʒn]

美[ɛnˈvɪʒən]

vt. 想像,预见,展望;

[例句]In the future we envision a federation of companies

我们设想将来会成立一个公司联盟。

[其他] 第三人称单数:envisions 现在分词:envisioning 过去式:envisioned 过去分词:envisioned





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