Yanzy的个人博客分享 http://blog.sciencenet.cn/u/Yanzy

博文

粗山羊草基因组揭示了转座子的多重影响

已有 1871 次阅读 2017-11-23 12:42 |系统分类:论文交流

本文于2017年11月20日在线发表在Nature Plants期刊上,原题目为:

The Aegilops tauschii genome reveals multiple impacts of transposons


摘要:

小麦是一种重要的全球作物,其基因组中含有比任何其他已知作物种类更多的转座因子(TEs)。在这里,我们构建了小麦D基因组供体粗山羊草的染色体规模,高质量的基因组,其中92.5%的序列已经锚定在染色体上。以此,我们准确地表征基因位点,基因表达,假基因,甲基化,重组率,microRNAs,特别是在染色体上的TEs。除了发现最近一次的基因复制,我们还发现了大约一半的基因发生了TE,发现这些基因的表达水平低于没有TE的基因,可能是因为它们的甲基化水平升高。我们绘制了所有小麦分子标记,并构建了与基因组序列相对应的高分辨率整合遗传图谱,从而将以前检测到的农业上重要的基因/数量性状基因座(QTL)第一次置于粗山羊草基因组上。


Abstract

Wheat is an important global crop with an extremely large and complex genome that contains more transposable elements (TEs) than any other known crop species. Here, we generated a chromosome-scale, high-quality reference genome of Aegilops tauschii, the donor of the wheat D genome, in which 92.5% sequences have been anchored to chromosomes. Using this assembly, we accurately characterized genic loci, gene expression, pseudogenes, methylation, recombination ratios, microRNAs and especially TEs on chromosomes. In addition to the discovery of a wave of very recent gene duplications, we detected that TEs occurred in about half of the genes, and found that such genes are expressed at lower levels than those without TEs, presumably because of their elevated methylation levels. We mapped all wheat molecular markers and constructed a high-resolution integrated genetic map corresponding to genome sequences, thereby placing previously detected agronomically important genes/quantitative trait loci (QTLs) on the Ae. tauschii genome for the first time.






https://blog.sciencenet.cn/blog-634883-1086479.html

上一篇:一种在植物和哺乳动物细胞中高效的由Cas9驱动的基因激活
下一篇:一种非典型的CCCH-串联锌指蛋白抑制了水稻次生壁的合成并控制...
收藏 IP: 114.251.216.*| 热度|

0

该博文允许注册用户评论 请点击登录 评论 (0 个评论)

数据加载中...
扫一扫,分享此博文

Archiver|手机版|科学网 ( 京ICP备07017567号-12 )

GMT+8, 2024-4-26 23:43

Powered by ScienceNet.cn

Copyright © 2007- 中国科学报社

返回顶部