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Stimulated emission depletion (STED) microscopy图像:
看看线粒体:线粒体在一般光学显微镜下就是一个小点或短棒,在共聚焦下(B)仍然是模糊的,但在STED下,哈!
STED microscopy enables the analysis of submitochondrial protein distributions. (A) Mitochondria form a branched network within eukaryotic cells. The mitochondria (green) and the microtubule cytoskeleton (red) of PtK2 cells were labeled with antibodies specific for Tom20 and α-tubulin. The nuclei were labeled with DAPI (blue). (Scale bar: 20 μm.) (B) Mitochondria of PtK2 cells labeled with an antiserum against Tom20. STED microscopy (Right) reveals individual Tom20 clusters, which are blurred and not resolvable when using diffraction-limited confocal microscopy (Left). (Scale bar: 2 μm.)
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