ABBS博客分享 http://blog.sciencenet.cn/u/chshou 自由的小鱼

博文

ABBS: Triticumoside induces apoptosis via caspase-dependen

已有 1731 次阅读 2016-6-2 10:33 |个人分类:期刊新闻|系统分类:论文交流| pathway, caspase, Triticum, aestivum

Triticumoside induces apoptosis via caspase-dependent mitochondrial pathway and inhibits migration through downregulation of MMP2/9 in human lung cancer cells

Barun Poudel,  Hyeon-Hui Ki,  Bui Thi Thuy Luyen,  Young-Mi Lee,  Young-Ho Kim and  Dae-Ki Kim

Department of Immunology and Institute for Medical Sciences, Chonbuk National University Medical School, Jeonju, Jeonbuk 561-756, Republic of Korea  
Acta Biochim Biophys Sin 2016, 48: 153–160; doi: 10.1093/abbs/gmv124

Non-small cell lung cancer (NSCLC) is the major cancer-related death worldwide with only 14% five-year survival rate. Triticumoside, a phenolic compound present in Triticum aestivum sprout extract, has been recognized to have antiobesity and anti-inflammatory effects. However, the effect of triticumoside on cancer cell proliferation and migration has not been studied. In order to elucidate whether triticumoside exhibits an anticancer effect, cells were incubated with different doses of triticumoside, and apoptosis was assessed by observing cell viability, cellular morphological changes, and annexin-V-fluorescein isothiocyanate/propidium iodide staining. Cell cycle analysis, western blotting, wound healing assay, and quantitative-polymerase chain reaction were also performed. Triticumoside exhibited marked cytotoxicity in the cells in dose- and time-dependent manner. Triticumoside caused morphological changes, including cellular rounding, nuclear condensation, and shrinkage. Likewise, triticumoside enhanced the sub-G1 proportion of cells. Additionally, triticumoside regulated expression of apoptosis-associated proteins, such as B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X, and procaspase-3/9. Triticumoside also inhibited migration of the cells through downregulation of matrix metalloproteinase-2/9 (MMP2/9). Collectively, these results suggest that triticumoside induces apoptosis through caspase-dependent mitochondrial pathway and suppresses migration via inhibition of MMP2/9 in NSCLC A549 cells.

Effect of triticumoside on A549 cells migration

阅读原文: http://www.abbs.org.cn/arts.asp?id=3975 

相关论文:



https://blog.sciencenet.cn/blog-592748-981906.html

上一篇:ABBS: Angiotensin II induces an increase in MMP-2 expression
下一篇:ABBS: Computational allosteric ligand binding site identific
收藏 IP: 202.127.20.*| 热度|

0

该博文允许注册用户评论 请点击登录 评论 (0 个评论)

数据加载中...
扫一扫,分享此博文

Archiver|手机版|科学网 ( 京ICP备07017567号-12 )

GMT+8, 2024-3-29 20:41

Powered by ScienceNet.cn

Copyright © 2007- 中国科学报社

返回顶部