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ABBS: SLC39A7 (Zip7) in human colorectal cancer

已有 722 次阅读 2019-5-23 09:15 |个人分类:期刊新闻|系统分类:论文交流| SLC39A7, Zip7, Zinc transporter, colorectal cancer

Knockdown of SLC39A7 inhibits cell growth and induces apoptosis in human colorectal cancer cells

Nengquan Sheng, Li Yan, Weiqiang You, Gewen Tan, Jianfeng Gong, Hongqi Chen, Yi Yang, Landian Hu, and Zhigang Wang

Department of General Surgery, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai 200233, China

Acta Biochim Biophys Sin 2017, 49: 926–934; doi: 10.1093/abbs/gmx094

SLC39A7 (zip7) is a zinc transporter that plays a key role in intestinal epithelial self-renewal. However, little is known about SLC39A7 in colorectal cancer. To assess the biological function of SLC39A7 in colorectal cancer, the expression of SLC39A7 in human colorectal tumors and five colorectal cancer cell lines were evaluated by Oncomine Cancer Microarray Database and western blot analysis. In addition, short hairpin RNAs specifically targeting SLC39A7 were transfected into HCT116 and SW1116 cells to knockdown SLC39A7 expression. Then, the effects of SLC39A7 knockdown on colorectal cancer cells were detected by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide, colony-forming assay and flow cytometry. Our results showed that colorectal tumors have higher expression levels of SLC39A7 than normal colon tissues. Knockdown of SLC39A7 exhibited a significant decrease in cell viability and proliferation of colorectal cancer cells. It was also shown that knockdown of SLC39A7 interfered with cell cycle progression and induced G2/M cell cycle arrest, as well as boosted early and late apoptosis in colorectal cancer cells. Furthermore, downregulation of SLC39A7 promoted the cleavage of PARP and enhanced the expression of Bad, Caspase-9, and cleaved-Caspase-3, as well as suppressed Bcl-2 expression. In conclusion, our results suggest that SLC39A7 plays a crucial role in the proliferation and survival of colorectal cancer cells, which associates with colorectal tumorigenesis.


Knockdown of SLC39A7 attenuated proliferation and colony formation of colorectal cancer




1 An essential role for the Zn2+ transporter ZIP7 in B cell development

2 Discovery of a ZIP7 inhibitor from a Notch pathway screen

3 Analysis the prognostic values of solute carrier (SLC) family 39 genes in gastric cancer

4 The Zinc Transporter SLC39A7 (ZIP7) Is Essential for Regulation of Cytosolic Zinc Levels

5 Requirement of Zinc Transporter SLC39A7/ZIP7 for Dermal Development to Fine-Tune Endoplasmic Reticulum Function by Regulating Protein Disulfide Isomerase

6 Zinc Transporter SLC39A7/ZIP7 Promotes Intestinal Epithelial Self-Renewal by Resolving ER Stress

7 Zinc transporters and cancer: a potential role for ZIP7 as a hub for tyrosine kinase activation

8 ZIP7-mediated intracellular zinc transport contributes to aberrant growth factor signaling in antihormone-resistant breast cancer cells


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