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Research article: 土壤动物跳虫加速了抗性基因的转移

已有 433 次阅读 2019-6-11 19:28 |个人分类:SEL进展|系统分类:论文交流

细菌抗生素抗性已经成为人类健康的主要威胁。抗生素抗性基因的扩散和迁移已经引起人们的广泛关注。中科院城市所朱永官教授团队利用定量PCR方法及16S rRNA基因测序分析了跳虫在抗性基因转移中的作用,以及抗性基因转移与微生物群落变化的关系。

Abstract  Soils have become an important sink for antibiotic resistance genes (ARGs). To better understand the impacts of ARGs on the soil ecosystem, the transport of ARGs is a basic question. So far, however, the role of soil animals in the dispersal of ARGs is not understood. Here, two treatments (without collembolans and with collembolans) were established, each treatment included unamended and manure-amended soil, and soil samples were collected at 14, 28 and 56 days after incubation. The effects of the collembolan Folsomia candida on dispersal of ARGs in the soil ecosystem were explored using high-throughput qPCR combined with Illumina sequencing. As the culture time increased, more shared ARGs and OTUs were detected between the unamended and manured soil, especially in the treatment with collembolans. Vancomycin, aminoglycoside and MLSB genes may have been more readily transported by the collembolan. On the 28th day after incubation, a high abundance of mobile genetic elements (MGEs) was found in the treatment with collembolans. These results clearly reveal that collembolans can accelerate the dispersal of ARGs in the soil ecosystem. Procrustes analysis and the Mantel test both indicate that soil bacterial communities were significantly correlated with ARG profiles. Furthermore, partial redundancy analysis indicates that soil bacterial communities can explain 41.28% of the variation in ARGs. These results suggest that the change of soil microbial community have an important contribution to the dispersal of ARGs by the collembolan。

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