沉闷科学的掘墓人分享 http://blog.sciencenet.cn/u/Bearjazz

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每日翻译20190728

已有 398 次阅读 2019-7-28 18:57 |个人分类:翻译作品|系统分类:科研笔记| 昆虫, 无损, DNA提取

#编者信息

熊荣川

明湖实验室

xiongrongchuan@126.com

http://blog.sciencenet.cn/u/Bearjazz

Nondestructive DNA extraction was   performed using a Qiagen DNeasy kit. Whole specimens were soaked overnight in   extraction buffer at 37°C, with small perforations made to the side of the   abdomen and DNA extracted from the supernatant. Damage to specimens (some of   them to be holotypes in future species descriptions) was minimal, and   softness of specimens was maintained if transferred to 70% ethanol after the   extraction. Vouchers will be maintained as pinned dry specimens in the W. D. Sumlin   personal collection.

使用Qiagen DNeasy试剂盒进行非破坏性DNA提取。在标本腹部侧面形成小孔,整个标本在37°C的提取缓冲液中浸泡一整夜,然后从上清液中提取DNA。对标本(其中一些在未来物种描述中是正模标本)的损害是最小的,如果提取后转移到70%的乙醇中,标本的柔软度保持不变。凭证标本将进行插针干燥,由W.D.Sumlin个人保管。

Pons J, Barraclough T G, Gomez-Zurita J, et   al. Sequence-based species delimitation for the DNA taxonomy of undescribed   insects[J]. Systematic biology, 2006, 55(4): 595-609.




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