沉闷科学的掘墓人分享 http://blog.sciencenet.cn/u/Bearjazz

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每日翻译20190630

已有 383 次阅读 2019-6-30 07:09 |个人分类:翻译作品|系统分类:科研笔记| 植物, 分子鉴定, DNAmarker, ITS序列成功率低

#编者信息

熊荣川

明湖实验室

xiongrongchuan@126.com

http://blog.sciencenet.cn/u/Bearjazz

For gymnosperms, we obtained high-quality   bidirectional sequences for the rbcL, matK, and trnH-psbA gene regions of the   chloroplast genome; we were able to obtain sequences for the ITS region from   all but one sample (Supporting Information Table S3). For angiosperms   (Supporting Information Table S3), PCR and sequencing success of the rbcL   gene region were the highest (99.37% and 98.74%, respectively). PCR success   of trnH-psbA was also high (97.05%), but sequencing success was lower   (92.02%). Substantially more effort was required to recover ITS sequences, which   had relatively high PCR success (96.01%) but sequencing success (89.50%) for   ITS was the lowest among the four gene regions, largely due to fungal   contaminants. Three pairs of primers were required for the recovery of matK,   but PCR and sequencing success were relatively low for this gene region   (93.07% and 92.02%, respectively).

对于裸子植物,我们获得了叶绿体基因组上rbcL, matKtrnH-psbA基因片段的高质量双向序列。对于ITS序列,除一个样本外(附表S3),其余样本都能获得该区域的序列。被子植物中(附表S3),rbcL基因片段的PCR和测序成功率最高(分别为99.37%98.74%)。trnH-psbAPCR成功率也很高(97.05%),但测序成功率稍低(92.02%)。总的来说,获取ITS序列需要更大的努力,因为它有较高的PCR成功率(96.01%),但测序成功率(89.50%)却是四个基因区域中最低的,主要是由于真菌污染。扩增matK需要三对引物,但该基因片段的PCR和测序成功率相对较低(分别为93.07%92.02%)。

Shao-Lin T , Ya-Huang L , Hollingsworth P   M , et al. DNA barcoding herbaceous and woody plant species at a subalpine   forest dynamics plot in Southwest China[J]. Ecology and Evolution, 2018




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