氢分子医学分享 http://blog.sciencenet.cn/u/孙学军 对氢气生物学效应感兴趣者。可合作研究:sunxjk@hotmail.com 微信 hydrogen_thinker

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氢气可保护放射诱导的免疫功能障碍

已有 1921 次阅读 2014-3-14 20:19 |个人分类:氢气生理盐水|系统分类:论文交流

放射线可导致机体损伤,其主要原因是放射线可导致身体内水和生物大分子被射线电离,形成自由基。放射性损伤也是自由基生物学最早研究的主体,因此可以说放射损伤是最典型的自由基损伤类型。虽然原子蛋爆炸在和平年代似乎可能性不大,但随着核能的广泛应用,人们接触反射线的机会在逐渐增加。日本311地震给日本民众带来的影响已经被广泛报道。另外,作为医学手段,人们长期使用放射线作为肿瘤治疗的方法。更不要说作为成像学的X线和PET所用的放射性核素了。总之,放射损伤无处不在。但是到目前人们对如何克服放射损伤的手段仍不理想,现有的药物都存在副作用大,效果有限等缺陷。因此寻找新的放射性药物一直是这个领域所注重的课题。身体不同组织对放射的敏感性有很大区别,一般来说,越是分裂活跃的细胞,对射线越敏感。例如首当其冲的是造血和免疫系统,然后是生殖细胞和肠道上皮细胞。纠正对放射诱导的免疫系统功能紊乱是对抗放射损伤的需要重点考虑的问题之一。

第二军医大学放射医学近年对氢气对抗放射损伤十分关注,已经发表相关论文近20篇,是国际上氢气对抗放射损伤的主要研究单位。最近该课题组再次发表相关研究论文,证明腹腔注射氢气生理盐水能保护放射诱导的动物免疫功能紊乱。

该论文发表在JCell Mol Med。研究主要从脾脏组织学、生物化学测定氧化应激相关分子如SODGSHMDA和自由基水平,流式细胞学技术测定细胞凋亡相关指标和分子,PCR监测T 细胞特异性标记分子的基因表达水平。结果发现,预先用氢气盐水可对抗放射诱导的脾脏组织损伤,同时各类氧化相关指标发生相应改变,细胞凋亡数量明显减少,T细胞特异性基因表达提高。研究说明氢气作为一种有效的放射损伤防护剂能通过清除自由基纠正放射诱导的免疫功能紊乱。

2014 Mar 12. doi: 10.1111/jcmm.12245. [Epub ahead of print]
Protective effect of hydrogen-rich saline against radiation-induced immune dysfunction.
Abstract

Recent studies showed that hydrogen can be used as an effective radioprotective agent through scavenging free radicals. This study was undertaken to evaluate the radioprotective effects of hydrogenon immune system in mice. H2 was dissolved in physiological saline using an apparatus produced by our department. Spleen index and histological analysis were used to evaluate the splenic structural damage. Spleen superoxide dismutase, GSH, MDA were measured to appraise the antioxidant capacity and a DCF assay for the measurement of radical oxygen species. Cell apoptosis was evaluated by an Annexin V-FITC and propidium iodide staining method as well as the apoptotic proteins such as Bcl-2, Bax, caspase-3 and c-caspase-3. CD4+ and CD8+ T cells subtypes were detected by flow cytometry with FITC-labelled antimouse CD4 and PE antimouse CD8 staining. Real-time PCR was utilized to determine the CD4+ T cell subtypes and related cytokines. Our study demonstrated that pre-treatment with H2could increase the spleen index and attenuate the radiation damage on splenic structure. Radical oxygen species level was also reduced by H2 treatment. H2 also inhibited radiation-induced apoptosis in splenocytes and down-regulated pro-apoptotic proteins in living mice. Radiation-induced imbalance of T cells was attenuated by H2 . Finally, we found that H2 could regulate the polarization of CD4+ T cells and the level of related cytokines. This study suggests H2 as an effective radioprotective agent on immune system by scavenging reactive oxygen species.



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