氢分子医学分享 http://blog.sciencenet.cn/u/孙学军 对氢气生物学效应感兴趣者。可合作研究:sunxjk@hotmail.com 微信 hydrogen_thinker

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氢气生理盐水对脊髓缺血的治疗作用

已有 2890 次阅读 2013-4-18 11:23 |个人分类:氢气生理盐水|系统分类:科研笔记|关键词:氢气,治疗| 氢气, 治疗

来自国内多家单位合作的一项关于氢气生理盐水治疗脊髓缺血再灌注损伤的研究,最近在线发表在《脑研究》。过去曾经有报道呼吸氢气对这一疾病治疗效果的研究,本研究采用注射氢气生理盐水的方法,再次验证了氢气对脊髓缺血的治疗效果。

脊髓缺血是临床上大血管手术中非常常见并发症,由于大血管手术,特别是主动脉手术过程中必须阻断血流,而所支配的下肢、腹腔器官和脊髓都可能受到缺血再灌注损伤,而脊髓对缺血最为敏感,因此是非常常见的临床问题。当然在脊髓创伤和脊髓血栓形成也可以发生脊髓缺血损伤。

本研究采用兔脊髓缺血再灌注损伤模型,采用静脉注射氢气生理盐水的方法,并对线颗体、氧化损伤、炎症反应和细胞凋亡等方面的指标进行了观察,结果发现,氢气生理盐水治疗可以有效治疗脊髓缺血损伤。提示氢气生理盐水在脊髓损伤上具有潜在应用价值。

Beneficial effects of hydrogen-rich saline against spinal cord ischemia-reperfusion injury in rabbits

Leshun Zhoua, 1, Xiaowu Wangb, 1, Weining Xuec, d, 1, Keliang Xiee, Yi Huangf, Hongguang Chene, Gu Gonga, , , Yi Zengf, , a Department of Anesthesiology, General Hospital of Chengdu Military Command, Chengdu 610083, Sichuan Province, P. R. China

b Centre of Cardiovascular Surgery, Guangzhou General Hospital, Guangzhou Military Command, Guangzhou 510010, Guangdong Province, P. R. China

c Department of Neurosurgery, Tangdu Hospital, Fourth Military Medical University, Xi'an, 710038, Shaanxi Province, P. R. China

d Department of First Surgery, No. 22 Hospital of PLA, Geermu 816000, Qinghai Province, P. R. China

e Tianjin Institute of Anesthesiology, Department of Anesthesiology, General Hospital of Tianjin Medical University, Tianjin 300052, P. R. China

f Department of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, Shaanxi Province, P. R. China

Accepted 10 April 2013Available online 17 April 2013

http://dx.doi.org/10.1016/j.brainres.2013.04.007, How to Cite or Link Using DOIPermissions & Reprints

Highlights

Hydrogen-rich saline is beneficial to spinal cord ischemia-reperfusion injury.

Activation of mitoKATP channels contributes to the protection of hydrogen-rich saline.

Hydrogen-rich saline can reduce oxidative stress, inflammatory cytokines and apoptosis.

Abstract

Hydrogen-rich saline (HS) is reported to be a new therapeutic agent in ischemia-reperfusion (I/R)-induced organ damage. The present study was designed to investigate the beneficial effects of HS against spinal cord I/R injury and its associated mechanisms. Spinal cord ischemia was induced by infrarenal aortic occlusion for 20 min in male New Zealand white rabbits. Different doses of HS were intravenously (i.v.) administered at 5 min before or after the beginning of reperfusion. Moreover, the roles of mitochondrial ATP-sensitive potassium channels (mitoKATP), oxidative stress, inflammatory cytokines and apoptosis was assessed. Here, we found that I/R-challenged rabbits exhibited significant spinal cord injury characterized by the decreased numbers of normal motor neurons and hind-limb motor dysfunction, which was significantly ameliorated by 5 mL/kg and 10 mL/kg HS treatment before reperfusion or 10 mL/kg HS treatment after reperfusion. However, the protective effects of HS treatment in spinal cord I/R injury were partially abolished by the selective mitoKATP channel blocker 5-hydroxydecanoate (5-HD). Moreover, we showed that the beneficial effects of 10 mL/kg HS treatment against spinal cord I/R damage were associated with the decreased levels of oxidative products [8-iso-prostaglandin F2α (8-iso-PGF2α) and malondialdehyde (MDA)] and pro-inflammatory cytokines [tumor necrosis factor-alpha (TNF-α) and high-mobility group box 1 (HMGB1)], as well as the increased activities of antioxidant enzymes [superoxide dismutase (SOD) and catalase (CAT)] in serum at 6 h, 12 h, 24 h, 48 h and 72 h after reperfusion and in spinal cord at 72 h after reperfusion. Furthermore, HS treatment (10 mL/kg) reduced caspase-3 activity in the spinal cord of this model. Thus, HS may be an effective therapeutic agent for spinal cord I/R injury via activation of mitoKATP channels as well as reduction of oxidative stress, inflammatory cytokines and apoptosis.



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