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氢气可对抗水稻种子发芽过程的盐胁迫

已有 3515 次阅读 2013-2-11 12:49 |个人分类:植物氢气效应|系统分类:论文交流|关键词:3,namespace,office,style| office, style, namespace

Hydrogen-rich water alleviates salt stress in rice during.pdf

 

来自南京农业大学生命科学院沈文飚教授课题组的关于氢气植物效应的研究,最近在线发表在Plant Soil。这是该课题组最近连续发表的第三篇关于氢气提高植物抗性研究的系列文章,进一步奠定了我国学者在氢气植物效应研究领域的地位。

http://link.springer.com/article/10.1007%2Fs11104-013-1614-3

本研究的目的是研究氢气增强水稻种子的萌发对盐胁迫的耐受性的分子机制。研究结合的生理和分子生物学方法,氢气饱和水HRW对盐胁迫的缓解作用。结果发现,100 mMNaCl胁迫引起水稻发芽种子释放H2增加,外源性氢气饱和水对100 mMNaCl引起的种子萌发幼苗生长抑制有对抗作用。进一步研究表明,两种50%和100%浓度外源性氢气饱和水能激活的α/β-淀粉酶的活性,从而加速形成的还原糖和可溶性总糖。总酶活性、isozymatic活性和抗氧化酶转录活性超氧化物歧化酶、过氧化氢酶和抗坏血酸过氧化物酶等都明显也增强。氧化损伤相关指标明显降低。此外,地上部和根系部分钾钠比率增加。总之,结果表明,外源性氢气处理对水稻种子可能是一个好的手段​​,可以减轻盐分胁迫。

文章回顾了早期关于氢气在植物生理学的研究文献Boichenko 1947; Sanadze 1961; Renwick et al. 1964

Since 1947, there have been several reports of H2 evolution and uptake in illuminated leaves, germinating seeds, and isolated chloroplasts (Boichenko 1947; Sanadze 1961; Renwick et al. 1964). More recently, our report illustrated the participation of H2 in enhancing salt tolerance in Arabidopsis seedlings (Xie et al. 2012). Exposure of alfalfa seedlings to paraquat stress increased endogenous H2 production, and H2 was able to act as a novel bioactive molecule in enhancing plant tolerance to paraquat-induced oxidative stress (Jin et al. 2012). In agreement with these, we also observed that NaCl stress caused an increase in H2 production in rice seedlings, although we did not investigate the enzymatic resources of H2 release. Thus, this result raised a significant question: if possible, what is the importance of this increased H2 production?

1.         Boichenko EA (1947) Hydrogenase from isolated chloroplasts. Biokhimiya 12:153–162

2.         Sanadze GA (1961) Absorption of molecular hydrogen by green leaves in light. Fiziol Rast 8:555–559

3.         Renwick GM, Giumarro C, Siegel SM (1964) Hydrogen metabolism in higher plants. Plant Physiol 39:303–306. doi:10.1104/pp.39.3.303

Hydrogen-rich water alleviates salt stress in rice during seed germination

Abstract
Aims

This study investigated the molecular mechanism of hydrogen-rich water (HRW)-mediated enhancement of tolerance against salinity stress during rice seed germination.

Methods

A combination of physiological and molecular approaches was used to study the effect of HRW on the alleviation of salinity stress.

Results

A 100-mM NaCl stress caused the increase of H2 release in germinating rice seeds. With respect to samples treated with 100 mM NaCl alone, exogenous HRW pretreatments differentially attenuated the inhibition of seed germination and seedling growth caused by salinity. Further results showed that both 50 % (in particular) and 100 % concentration of HRW could activate α/β-amylase activity, thus accelerating the formation of reducing sugar and total soluble sugar. HRW also enhanced total, isozymatic activities or corresponding transcripts of antioxidant enzymes, including superoxide dismutase, catalase, and ascorbate peroxidase. These results were confirmed by the alleviation of oxidative damage, as indicated by a decrease of thiobarbituric acid reactive substances. Additionally, the ratio of potassium (K) to sodium (Na) in both the shoot and root parts was increased.

Conclusions

Together, our results suggested that exogenous HRW treatment on rice seeds may be a good option to alleviate salinity stress.



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