一氧化碳中毒可引起大脑损伤，比较典型的是一氧化碳中毒后迟发性脑病，一氧化碳中毒后迟发性脑病非常类似于神经自身免疫性疾病，如脱髓鞘的多发性硬化或广泛脑挫伤，但这类疾病缺乏非常有效的治疗手段，往往给患者和家庭造成严重的影响。

我们实验室曾经报道过氢气对一氧化碳中毒后迟发性脑病的研究，第四军医大学也曾经报道过同样的效应，最近他们在这个基础上，通过对大脑神经炎症反应的观察和电子显微镜结构分析，再次证明氢气对这一疾病的治疗价值。文章发表在Neurol Res.

Hydrogen rich saline reduces immune-mediated brain injury in rats with acute car.pdf

Neurol Res. 2012 Dec;34(10):1007-15. doi: 10.1179/1743132812Y.0000000106.

Hydrogen rich saline reduces immune-mediated brain injury in rats with acute carbon monoxide poisoning.

Wang W, Li Y, Ren J, Xia F, Li J, Zhang Z.

School of Aerospace Medicine, Fourth Military Medical University, Xi'an, China.

This experiment was designed to determine whether hydrogen (H(2)) rich saline can ameliorate brain abnormalities in a rat model with acute carbon monoxide (CO) poisoning.

Sprague-Dawley male rats were used for CO poisoning and H(2) rich saline treatment. Changes in neurons, microglias, and myelin sheath were observed by electron microscope. Neuron loss was assessed by Nissl staining. Antioxidant capacities were evaluated by studying superoxide dismutase activities and malondialdehyde concentration in the brain and serum. Infiltration of macrophages, expression of immune-associated cytokines (MIP-1-alpha and ICAM-1), and changes in myelin basic protein (MBP) were monitored by immunohistochemical staining and western blotting.

CO-exposed rats showed the increase in neuron loss and the decrease in antioxidant capacities. And H(2) rich saline given after CO poisoning can prevent the alterations mentioned above. CO-mediated oxidative stress caused alterations in MBP, which initiated an adaptive immunological response that led to brain injury. MBP from H(2) rich saline-treated, CO-exposed rats was recognized normally by immunohistochemical staining and western blotting. Electron microscope observation from CO-exposed rats showed an apparent aggregation of microglias. Macrophages from CO-exposed rats were significantly more than those from H(2) rich saline-treated and control rats, and the immunofluorescence observation showed that macrophages were similar to microglias in type. Expression levels of MIP-1-alpha and ICAM-1 increased in the brains of CO-poisoned rats and H(2) rich saline treatment decreased the levels.

The results indicate that H(2) rich saline prevents immune-mediated brain injury after CO poisoning.

PMID: 23146304 [PubMed - in process]