纳米铂是纳米尺度的金属铂颗粒，由于具有非常良好的比表面积，结合铂的催化活性，研究发现该物质具有良好的抗氧化活性。氢气和纳米铂联合具有更理想的抗氧化作用，过去曾经发现两者联合使用具有抗肿瘤作用，本研究主要从红细胞氧化损伤和细胞膜完整性以及血液流动性等角度观察氢气和纳米铂的协同效应。

聚乙烯吡咯烷酮（可作为血浆代用品）包埋的纳米铂可以分散到水中，也可以分散到富氢水中(氢气浓度0.82 ppm; 氧化还原电位-583mv，没有饱和？或者氢气的溶解度本来就这样低)。本研究评价纳米铂分散富氢水和纳米铂水对1，1-二苯基-2-三硝基苯肼(DPPH)自由基的清除能力，以及对2,2'-偶氮(2-脒基丙烷)二氯化氢(AAPH)诱导的氧化应激后血液流动性的影响。使用电子自旋共振技术研究发现，当纳米铂浓度达到0.25-0.5 ppm时，正常水和氢气水使DPPH自由基可分别下降77.5-59.6% 或16.1-5.6%，如果没有纳米铂，氢气水只可以使DPPH自由基下降到81.3%（这个结果说明纳米铂具有清除自由基的作用，，尽管氢气本身也有一定作用，但氢气和纳米铂联合可以大大促进这种作用，也就是说具有协同作用）。

采用AAPH诱导氧化应激，将马血和正常水或氢气水共同孵育24小时后，马血通过血液流变仪微隧道的时间分别为13.7 sec (100%) 或 5.7 sec (42.3%)。当联合用0.5-1.0 ppm纳米铂时，马血通过血液流变仪微隧道的时间分别为9.7-7.3 sec (71.6-53.8%)或4.3-1.3 sec (32.8-10.3%)。（和抗自由基的作用方式类似）。红细胞聚集的比例从无纳米铂时的42.8%下降到23.8-21.0% or 15.8-9.8%,。使用电子扫描显微镜研究发现，AAPH诱导氧化应激可使红细胞膜失去完整性，伴随细胞膜无中空突起，显示红细胞的膜流动性受损。红细胞聚集的比例达到46.2%。氢气水可以使红细胞聚集的比例降低到29.6%。而1.0 ppm纳米铂可以使使红细胞聚集的比例降低到24.1% ，氢气水和1.0 ppm纳米铂联合作用为21.1%。

研究结果现实，氢气和纳米铂具有协同效应，可以联合使用于氧化应激类疾病的治疗。

J Nanosci Nanotechnol 2012 May;12 (5): 4019-27. [IF:1.352]

Colloidal platinum in hydrogen-rich water exhibits radical-scavenging activity and improves blood fluidity.

Kato S , Hokama R , Okayasu H , Saitoh Y , Iwai K , Miwa N .

Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima, Nanatsuka 562, Shobara, Hiroshima 727-0023, Japan.

Abstract

The 'colloidal platinum' stabilized with polyvinylpyrrolidone (Pt/PVP-colloid) was dispersed in hydrogen-rich water (HW; hydrogen concentration, 0.82 ppm; oxidation-reduction potential, -583 mV) or regular water (RW; <0.01 ppm, +218 mV). And we evaluated the antioxidant activity of Pt/PVP-colloid in HW or RW on 1,1-diphenyl-2-picrylhydrazyl  radical scavenging and improvement of blood fluidity under 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH)-induced oxidative stress.

When applied with the 0.25-0.5 ppm Pt/PVP-colloid in RW or HW, the level of DPPH radicals decreased to 77.5-59.6% or 16.1-5.6%, in contrast to the level as high as 81.3% for HW alone, respectively, as measured by an electron spin resonance method.

The horse blood, which was subjected to AAPH-induced oxidative stress, was incubated for 24 hr with RW or HW, and thereafter required 13.7 sec (100%) or 5.7 sec (42.3%) for passing through the micro-channels in a rheology equipment. When treated with 0.5-1.0 ppm Pt/PVP-colloid in RW or HW, the blood passage time in the micro-channels decreased dose-dependently to 9.7-7.3 sec (71.6-53.8%) or 4.3-1.3 sec (32.8-10.3%), and the rate of micro-channels clogged with erythrocyte aggregates decreased to 23.8-21.0% or 15.8-9.8%, respectively, from 42.8% for no addition of Pt/PVP. By scanning electron microscopy, AAPH-treated erythrocytes lost intact surface morphology on the membrane together with protrusions and without hollows, being indicative of impaired transforming ability, and the rate of erythrocyte agglutination was increased to 46.2%. When treated the horse blood with HW alone significantly decreased the rate of erythrocyte agglutination to 29.6%, whereas 1.0 ppm Pt/PVP-colloid in RW or HW decreased it to 24.1% or 21.1%, respectively. Thus, DPPH-radical-scavenging and erythrocyte-protecting effects of Pt/PVP-colloid in HW were superior to those of Pt/PVP-colloid in RW or Pt/PVP-free HW. The results could be mainly attributed to the enhanced antioxidant activity of Pt/PVP in HW, which may be due to captured-hydrogen on platinum.

PMID: 22852342 [Pubmed - In-Process]