动脉粥样硬化是以大血管泡沫细胞脂肪沉积为特征的进展性疾病。氧化的低密度脂蛋白在动脉粥样硬化发生发展中发挥重要作用，而氧化的低密度脂蛋白是由于氧化应激引起，随后导致内皮细胞激活和炎症反应，并导致炎症细胞浸润，巨噬细胞吞噬低密度脂蛋白，变成泡沫细胞。尽管对具体机制不清楚，但研究表明许多引起氧化应激的危险因素如吸烟、糖尿病、高血脂、高血压和牙周病等都是引起动脉硬化的重要因素。牙周病是一种常见炎症性疾病，损伤周围可以产生过量活性氧，许多研究认为牙周病和心血管疾病关系密切，和动脉硬化的发生存在相关关系。有研究发现，牙周病可导致系统活性氧和氧化低密度脂蛋白增加，根据这个发现，使用抗氧化治疗可作为干预牙周病引起的动脉粥样氧化的手段。

氢气分子的选择性抗氧化作用发现后，作为一种新型的抗氧化物质，通过饮用氢气水对动脉硬化具有治疗作用已经被证明。那么氢气水可否具有阻断牙周病诱导的动脉硬化值得探讨。

这个研究假定氢气可以降低血液中氧化低密度脂蛋白，阻断牙周病诱导的动脉氧化应激，从对动脉硬化具有治疗作用。

本研究使用乙酰赖氨酸（hexanoyl-lysine (HEL)）作为早期氧化指标、硝基酪氨酸（蛋白硝基化）和8-OHdG（核酸氧化指标）作为评价动脉氧化应激的指标。血清活性氧代谢产物reactive oxygen metabolites (ROM)作为循环系统氧化应激的指标。

局部组织动脉硬化相关指标，牙齿周围病理改变，动脉硬化形态学观察。研究结果发现，氢气水对牙周病及其诱导的动脉硬化均有显著改善效果。提示氢气水可能可作为该类疾病的治疗手段。

Hydrogen-rich water prevents lipid deposition in the.pdf

Hydrogen-rich water prevents lipid deposition in the descending aorta in a rat periodontitis model

•      Daisuke Ekunia, •   Takaaki Tomofujia,  ,  ,

•     a Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan

•      http://dx.doi.org/10.1016/j.archoralbio.2012.04.013, How to Cite or Link Using DOI

Objective

Periodontitis has been causally linked to atherosclerosis, which is mediated by the oxidative stress. As hydrogen-rich water (HW) scavenges reactive oxygen species (ROS), we hypothesized that HW could prevent lipid deposition induced by periodontitis in the aorta. The aim of this study was to investigate the effects of HW on the initiation of atherosclerosis in a rat periodontitis model.

Design

Eighteen 8-wk-old male Wistar rats were divided into three groups of six rats; the periodontitis group, periodontitis + HW group and the no treatment (control) group. In the periodontitis and periodontitis + HW groups, periodontitis was induced using a ligature for 4 wk, while the periodontitis + HW group was given water containing 800–1000 μg/L hydrogen during the 4-wk experimental period.

Results

In the periodontitis group, lipid deposition in the descending aorta was observed. The periodontitis group also showed significant higher serum levels for ROS and oxidised low-density lipoprotein-cholesterol (ox-LDL) (1.7 and 1.4 times, respectively), and higher aortic expression levels of nitrotyrosine and hexanoyl-lysine (HEL) (7.9 and 16.0 times, respectively), as compared to the control group (p < 0.05). In the periodontitis + HW group, lipid deposition was lower. Lower serum levels of ROS and ox-LDL (0.46 and 0.82 times, respectively) and lower aortic levels of nitrotyrosine and HEL (0.27 and 0.19 times, respectively) were observed in the periodontitis + HW group than in the periodontitis group (p < 0.05).

Conclusions

HW intake may prevent lipid deposition in the rat aorta induced by periodontitis by decreasing serum ox-LDL levels and aortic oxidative stress.

Abbreviations