氢水洗澡3个月可以显著减少皮肤皱纹。

Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ra.pdf

不久前曾经有韩国学者用动物试验证明《氢气洗澡能对抗紫外线》引起的动物皮肤损伤。本研究采用体外细胞学研究并进行了人体试验。使用温电解富氢气水，温度为41度，氢气的溶解量为1.13 ppm，氧化还原电位为-741 mV。普通水中氢气的含量为0.01 ppm以下（没有测定出），氧化还原电位为+184 mV。选择皮肤相关的纤维母细胞OUMS-36和角质细胞HaCaT用于紫外线损伤试验。结果发现，3-5天后氢水可以使OUMS-36细胞I型胶原合成增加1.85到2.03倍，免疫组织化学染色显示I型胶原主要分布在细胞核周围。细胞增殖及细胞毒性（WST-1）和细胞凋亡与坏死（Hoechst 33342）检测结果发现，氢气水可以显著保护HaCaT角质细胞的DNA损伤，如细胞核固缩、核酸片断形成等。氯化硝基四氮唑蓝（NBT）光化还原法证明氢气对两种细胞的超氧阴离子产生都具有抑制作用。

人体抗皱效应试验采用氢气水沐浴，6名日本人参加，每天使用氢气含量为0.2-0.4 ppm的水进行沐浴，连续3个月。通过第90天与试验前颈和背部皮肤的比较，发现其中4名受试者颈和背部皮肤皱纹明显减少。研究提示，氢气水洗可通过清除活性氧作为日常皮肤防紫外线辐射损伤和抗皱手段。

这是来自日本最新研究，文章发表在Journal of Photochemistry and Photobiology B: Biology。题目为Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-I collagen production and oxidative-stress diminishment in fibroblasts and cell-injury prevention in keratinocytes

来自日本广岛大学，生命与环境科学系的Shinya Kato^a, 1, Yasukazu Saitoh^a, 2, Keizou Iwai^b, Nobuhiko Miwa等作者认为，本研究的亮点为：通过试验证明，氢气水可促进纤维细胞胶原合成，清除自由基，抑制角质细胞死亡。临床研究证明氢气水沐浴具有抗皱效应。

本人评论，虽然这个研究非常有意思，毕竟是关于抗皮肤衰老首次进行的人体试验。但研究存在一些缺陷，细胞学试验证据不足，没有针对胶原蛋白合成的原因进行探讨，实际上皮细胞合成胶原的调节方式已经比较清楚，氢气如何影响这些途径是非常容易进行分析和研究的，至少要针对一些比较重要的信号分子进行检测。人体试验样本量太少，只有6个，而且只有4个有效，必须有10-20个人的数量才能算小样本的小型试验，这样的小规模试验无法证明这种手段的有效性。很难排除偶然性和人为因素的干扰。

英文摘要和目录

Hydrogen-rich electrolyzed warm water (HW) was prepared at 41°C and exhibited dissolved hydrogen (DH) of 1.13 ppm and an oxidation-reduction potential (ORP) of -741 mV in contrast to below 0.01 ppm and +184 mV for regular warm water (RW). Fibroblasts OUMS-36 and keratinocytes HaCaT were used to examine effects of HW against UVA-ray irradiation. Type-I collagen was synthesized 1.85- to 2.03-fold more abundantly by HW application for 3-5 days than RW in OUMS-36 fibroblasts, and localized preferentially around the nuclei as shown by immunostain. HW application significantly prevented cell death and DNA damages such as nuclear condensation and fragmentation in UVA-induced HaCaT keratinocytes as estimated by WST-1 and Hoechst33342 assays. HW significantly suppressed UVA-induced generation of intracellular superoxide anion radicals in both the cell lines according to NBT assay. Wrinkle repression was clinically assessed using a HW-bathing. Six Japanese subjects were enrolled in a trial of HW-bathing (DH, 0.2-0.4 ppm) every day for 3 months. HW-bathing significantly improved wrinkle in four subjects on the back of neck on 90^th day as compared to 0 day. Thus, HW may serve as daily skin care to repress UVA-induced skin damages by ROS-scavenging and promotion of type-I collagen synthesis in dermis.

Highlights

► Hydrogen-rich electrolyzed warm water (HW) promotes the type-I collagen synthesis in fibroblasts and suppresses cell death in keratinocytes, in accompanying with quenching UVA-derived intracellular reactive oxygen species. ► In addition, a clinical study of HW-bathing demonstrated the repressive-wrinkle formation on 90^th day as compared to 0 day. ► Therefore, continuous application of HW reduces oxidative stress in the skin, and expects to prevent the wrinkle formation.

Loader.rt("abs_end"); Loader.feature('lp_embed').qCode("loadEmbedContent(EMBED_APC, 'embedAPCModule');") Keywords: Hydrogen water; UV-A; Collagen type-I; Fluorescent immunostaining; Oxidative stress; Hydrogen water bathing; Wrinkle repression

Article Outline

1. Introduction

2. Materials and methods

2.1. Cell culture

2.2. Preparation of Hydrogen-rich Electrolyzed Warm Water (HW) and HW-containing medium

2.3. Application of Hydrogen-rich electrolyzed warm water (HW) and Immunostaining for type I collagen in OUMS-36 fibroblasts

2.4. Intracellular ROS-generation examined by NBT assay in OUMS-36 fibroblasts

2.5. Cell viability measured by WST-1 assay in relation to DNA -cleavage and intracellular ROS generation in HaCaT keratinocytes

2.6. Clinical evaluation of repressive-wrinkle formation by daily HW-bathing

2.7. Statistical analysis

3. Results

3.1. Effects of HW on type-I collagen production and antioxidant activity against UVA in OUMS-36 fibroblasts

3.2. Defensive effects of HW against UVA-induced injury in HaCaT keratinocytes

3.3. Clinical evaluation of repressive-wrinkle formation by HW-bathing

4. Discussion

5. Conclusions

References

Fig. 1. 

Parameters of hydrogen-rich electrolyzed warm water (HW) and regular warm water (RW) measured immediately after preparation and every 30 min up to 180 min of incubation at 37°C. Abbreviations represent Control: Reversed-osmosis ultra-pure water, HW: Hydrogen-rich electrolyzed warm water, RW: Regular warm water.