氢分子医学分享 http://blog.sciencenet.cn/u/孙学军 对氢气生物学效应感兴趣者。可合作研究:sunxjk@hotmail.com 微信 hydrogen_thinker

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氢气治疗肾脏缺血再灌注损伤

已有 6564 次阅读 2010-5-26 17:09 |个人分类:氢气生理盐水|系统分类:论文交流| 氢气

这个工作我们早就做出了结果,可惜由于论文写作相对比较慢,导致现在这种尴尬的局面,只能增加研究内容了。

实验的内容比较简单,缺血模型,一般病理染色,电子显微镜观察,结合肾脏功能检测和血液中氧化指标检测,成文发表,杂志分数3分。

 

Abstract
Purpose Renal ischemia–reperfusion (I/R), an important
cause of acute kidney injury, is unavoidable during various
types of operations, including renal transplantation, surgical
revascularization of the renal artery, partial nephrectomy,
and treatment of suprarenal aortic aneurysms.
Exacerbation of I/R injury is mediated by reactive oxygen
species (ROS). A recent study has shown that hydrogen has
antioxidant properties. In this study, we tested the
hypothesis that a hydrogen-rich saline solution (HRSS)
attenuates renal I/R injury in a rodent model.
Methods Rats were treated with an intravenous injection
of HRSS or control saline solution followed by renal I/R.
After 24 h of treatment, we performed a histological
examination and transmission electron microscopy, and
measured serum levels of 8-OHdG.
Results Histological analysis revealed a marked reduction
of interstitial congestion, edema, inflammation, and hemorrhage
in renal tissue harvested 24 h after HRSS treatment
compared to saline administration. Renal I/R injury, which
led to altered mitochondrial morphology, was also inhibited
by HRSS. Furthermore, serum 8-OHdG levels were
significantly lower in rats treated with HRSS and subjected
to renal I/R.

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