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氢气治疗急性胰腺炎

已有 7905 次阅读 2010-2-3 15:19 |个人分类:氢气生理盐水|系统分类:科研笔记|关键词:氢气| 氢气

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本文来自长征医院普通外科王强主任课题组,被BBRC接受。

急性胰腺炎目前最有效的治疗方法是用胰酶抑制剂,但费用非常昂贵,寻找更好的廉价治疗方法仍是临床关注的研究热点。本研究用含氢生理盐水治疗动物急性胰腺炎,用形态学方法证明能减轻胰腺组织损伤,减少NFKb的水平,减少细胞凋亡数量,并能促进胰腺腺细胞的增殖,采用淀粉酶和氧化损伤指标分别证明对胰腺功能和氧化损伤均有明显的改善作用,虽然研究的指标比较单薄。但本文首次在国际上证明氢气能有效治疗急性胰腺炎,为这一重要疾病的治疗提供了新有效手段的前景。希望将来在临床上能实现,将造福患者,降低费用。

Hydrogen-rich saline ameliorates the severity of L-arginine-induced acute pancreatitis in rats

ABSTRACT

Background and Aims: Acute pancreatitis is an inflammatory process involving pancreatic tissue edema, acinar cell damage, neutrophil infiltration and the rapid production and release of inflammatory cytokines, which play a dominant role in local pancreatic inflammation and systemic complications. Molecular hydrogen, which reacts with the hydroxyl radical, a highly cytotoxic species produced in inflamed tissues, has been considered as a novel antioxidant. The study was to evaluate the therapeutic effects of hydrogen-rich saline on the L-arginine(Arg)-induced acute pancreatitis.

Methods: Acute pancreatitis was induced in Sprague-Dawley rats by giving two intraperitoneal injections of Arg, each at concentrations of 250 mg/100 g body weight, with a 1 h interval between injections. Rats were divided randomly into three groups: Normal, Arg plus saline treatment, and Arg plus hydrogen-rich saline treatment groups. Hydrogen-rich saline ( > 0.6 mM, 6 ml/kg) or saline (6 ml/kg) was administered respectively via tail vein in 15 minutes after each Arg administration. Pancreatitis severity was assessed by analysis of serum amylase, pancreatic water content, and histology. Samples of pancreas were also taken for measuring malondialdehyde and myeloperoxidase. Apoptosis in pancreatic acinar cell was determined with terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling technique (TUNEL). Expression and distribution of proliferating cell nuclear antigen (PCNA) and nuclear factor kappa B ( NF-κB) were detected with immunohistochemistry.

Results: Hydrogen-rich saline treatment significantly attenuated the severity of Arg-induced acute pancreatitis with inhibiting Arg-induced apoptosis, pancreatic tissue edema, activation of NF-κB and promoting acinar cells proliferation. Moreover, Hydrogen-rich saline treatment significantly limited the neutrophil infiltration, lipid oxidation and ameliorated the increased serum amylase.

Conclusions: These results indicate that hydrogen treatment has a protective effect against acute pancreatitis. This protective effect is possibly due to its ability to inhibit oxidative stress, apoptosis and to promote acinar cell proliferation.

Keywords: Hydrogen, Acute pancreatitis, Antioxidant, Oxidative stress, Apoptosis

 目前已经发表的氢气生物学效应的论著统计,红色为中国论文,兰色为日本,黑色为美国

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