氢分子医学分享 http://blog.sciencenet.cn/u/孙学军 对氢气生物学效应感兴趣者。可合作研究:sunxjk@hotmail.com 微信 hydrogen_thinker

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氢气治疗视网膜缺血再灌注损伤

已有 5637 次阅读 2009-8-26 14:09 |个人分类:氢气生理盐水|系统分类:科研笔记|关键词:氢气,,氢分子医学| 氢气, 氢分子医学

从07年到现在,日本学者共发表相关文章12篇,其中日本医科大学的太田成男教授有11篇。几乎占这个领域的总数量的1半,仍是这个领域的第一。美国有三个大学开始这方面的研究,目前进展尽管比较快,但仍与日本的有一些差距。日本学者主要的方向是,脑、肝和心方面。美国的主要是小肠和肝。我们只在肺损伤方面有一些新意,其他在器官方面没有突出表现。唯一值得炫耀的是制备了含氢生理盐水,但并不是特别的创新。本文是除我们以外采用含氢生理盐水唯一的一篇论文。日本学者无论如何,每次都引用我们的文章,从这个方面看,太田成男教授对我们的研究是比较认可的。到目前,我们的第一篇这方面的文章引用次数已经超过10次(发表了1年),第二篇2009年发表的论文引用也已经达到8次。

这是采用局部用药的方法,证明氢气能治疗视网膜缺血再灌注损伤。特别指出,这个文献是作者直接发给我的。没有经过检索。特此谢谢。

这也说明他们一直在这方面开展一些工作,我们不能放松。

关于这个研究,实际上我们也开始,但采用的研究手段是不同的,研究结果也不如他们的理想。科研工作就是这样,尽管你能想到,甚至做到,但是如果晚了,就几乎是完了。

Abstract

PURPOSE. Retinal ischemia-reperfusion (I/R) injury by transient elevation of intraocular pressure (IOP) is known to induce neuronal damage through the generation of reactive oxygen species. Previous studies indicate that molecular hydrogen (H2) is an efficient antioxidant gas that selectively reduces the hydroxyl radical (•OH) and suppresses oxidative stress-induced injury in several organs. This study was conducted to explore the neuroprotective effect of H2-loaded eye drops on retinal I/R injury.

METHODS. Retinal ischemia was induced in rats by raising IOP for 60 minutes. H2-loaded eye drops were prepared by dissolving H2 gas into a saline to saturated level and administered to the ocular surface continuously during the ischemia and/or reperfusion periods. One day after I/R injury, apoptotic cells in the retina were quantified and oxidative stress was evaluated by markers such as 4-hydroxynonenal and 8-hydroxy-2-deoxyguanosine. Seven days after I/R injury, retinal damage was quantified by measuring the thickness of the retina. RESULTS. When H2-loaded eye drops were continuously administered, H2 concentration in the vitreous body immediately increased and I/R-induced •OH level decreased. The drops reduced the number of retinal apoptotic and oxidative stress marker-positive cells, and prevented retinal thinning with an accompanying activation of Müller glia, astrocytes, and microglia. The drops improved the recovery of retinal thickness by >70%. CONCLUSIONS. H2 has no known toxic effects on the human body. Thus, our study suggests that H2-loaded eye drops will be a highly useful neuroprotective and anti-oxidative therapeutic treatment for acute retinal I/R in

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