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我只是论文的搬运工 2019.5.15 海洋微藻鉴定与培养条件选择

已有 439 次阅读 2019-5-15 13:32 |个人分类:运粮工|系统分类:生活其它

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During a survey for marine microalgal resources, we isolated a rare marine euglenoid from the coastal waters of Qingdao, China in 2009, and established a pure culture. Electron microscopic and molecular phylogenetic (18S rDNA and 16S rDNA sequences) analyses revealed a close affifi nity with Eutreptiella gymnastica , a bloom-forming species. Different culture conditions were monitored to understand optimal E . gymnastica growth characteristics. The optimal growth conditions in a batch culture of this isolate were 20°C, 160 μmol photons/ (m 2×s) of white light, and a salinity of 10–31. Nutrient experiments demonstrated that growth increased dramatically with a phosphorus concentration greater than 72 μmol/L. Understanding the effect of culture conditions on E . gymnastica may help understanding the blooming mechanism of this alga in its natural environment.


 在对海洋微藻资源的调查中,我们于2009年从中国青岛沿海海域分离出一种稀有的海洋真藻类(眼虫藻纲),并进行了纯化培养。电子显微镜和分子系统生(18S rDNA16S rDNA序列)分析表明,其与一种开花的物种Eutreptiella gymnastica有密切的亲缘关系。对不同的培养条件进行了监测,了解E . gymnastica的最佳生长条件。这批分离培养的最适生长条件为:20°C160μmol光子/(m2×s)白光,盐度10-31。培养试验结果表明,当磷浓度大于72μmol/L时,生长显著加快。了解培养条件对E . gymnastica的影响,可能有助于了解这种藻类在自然环境中的开花机制。



参考文献:Molecular identififi cation and culture trials of Eutreptiella gymnastica (Eutreptiales, Euglenophyceae)



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