||
In vitro conversion of adult murine endothelial cells to hematopoietic stem cells
nature protocols 36.8 https://www.nature.com/articles/s41596-018-0060-3
【意义】体外产生造血干细胞对临床超级有用,外伤&癌症&先天疾病都用得着。帮助探索HSC发育以及自我更新机制理解。
【做了啥】本protocol就是阐述了把啮齿类的上皮细胞(ECs)转变成HSCs的步骤【rEC-HSPCs】(reprogrammed ECs into hematopoietic stem and progenitor cells')
【怎么做的】不经过转化成多能性状态,而是三步:induction(day0-8, 4个转录因子表达FGRS), specification(day 8-20, 上一步诱导了内源Runx1表达,因此命运特化), and expansion(day 20-28,血管niche使其扩增)
【区分】hepatocyte(hepatic) hematopoietic