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全球最高引100篇文章系统分析(1亿篇文章筛选出来,总共分7档) 精选

已有 6075 次阅读 2017-11-26 13:31 |个人分类:领域分析|系统分类:观点评述|关键词:PCR,BLAST,蛋白纯化,最高引用,100篇,文章,JBC,生物

iNature:对于全球最高引的前100篇文章,篇均引用量是34834.92次;这100篇文章主要聚集在生物化学及分子生物学领域;这100篇文章中,发表在PHYSICAL REVIEW B杂志及JBC杂志都是发表了7篇文章;对于这100篇文章,产出率最高的机构是加州大学;总的来说,这100篇文章主要是聚集在基础研究,如蛋白质的定量,蛋白质的分析,实时定量PCR技术,BLAST的诞生,DNA测序,同源序列比对等方面。iNature编辑组的统计时间是2017年11月26日。



1引用量最高的10000篇文章分析


我们以Web of Science为基础,通过相关的检索,从1874-2018年,总共检索到120611782条记录(图.1)。


470e0002839af4f240cc

图.1 总文章数


我们对于所有的文章进行了分类归档,发现引用次数大于10万次,为第一档,有3篇文章;第二档的引用量是5-10万次,有9篇文章;第三档的引用量是1-5万次,有238篇文章;第四档的引用量是5000-1万次,有748篇文章;第五档的引用量是1000-5000次,有22994篇文章;第六档的引用量是500-1000次,有68509篇文章;第七档的引用量是小于500次,有120519371篇文章图.2


文章分档引用次数文章数累加文章数
I>10万3
II5-10万912
III1-5万238250
IV5000-1万748998
V1000-50002299423902
VI500-10006850992411
VII<500120519371120611782

图.2 所有文章的归档分类



之后我们根据引用量从高到底,进行排序,我们筛选了10000条记录(因为Web of Science最多只能一次分析10000条记录),进行相应的统计分析。我们发现每篇文章的平均引用次数是3173.14次,总引用次数是31731393次,施引文献是14692585 图.3。另外,我们发现,最高引用文章大部分都聚集在1990-2010年之间,达到了40%以上。


470b00043757465e8043

图.3 10000篇文章引用分析


之后,我们看了一下不同时间段,对于这些文章的引用次数分析,发现绝大部分引用都是聚集在1990-2016年之间(图.4)。


470b0004382a0bb2e20d

图.4 10000篇文章不同时间段引用分析




由于分析10000篇文章,工作量太大,我们就直接分析前100篇文章,发现前100篇文章的平均引用量是34834.92次,总引用次数是3483492次,施引文献达到2627346篇。这100篇文章,占前10000篇的总引用的10.98%,但是总文章数是1.00%,故我们主要分析这100篇文章图.5



470e000286e795f6d3cc

图.5 100篇文章引用分析



2引用量最高的100篇文章分析


对于这100文章发表的时间进行分析,我们发现在1950年以前只有4篇,1950-1960年之间,有10篇;1961-1970年,有9篇;1971-2010年,总共有74篇,占了绝大部分;2010年以后,只有3篇文章(图.6),这三篇文章分别是遗传进化(MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods,2011年),癌症综述(Hallmarks of Cancer: The Next Generation,2011年),全球癌症统计(Global Cancer Statistics,2011年



470200048d6fcd4c3e01图.6 100篇文章发表时间分析


其次我们对这100篇文章的种类进行了基本分析,发现生物化学及分子生物学方面有35篇,物理学有15篇,化学有14篇,数学11篇图.7



470f0002143c752baf86

图.7 100篇文章方向分析


我们再次对这100篇文章发表的杂志进行分析(>3篇),发表在PHYSICAL REVIEW B杂志及JBC杂志都是7篇文章;ANALYTICAL BIOCHEMISTRY,JOURNAL OF CHEMICAL PHYSICS,NATURE,NUCLEIC ACIDS RESEARCH都是发表了4篇文章,总的来说,这高引用的100篇文章,大部分都是生物,物理,生物化学及化学方面的文章。另外这9个杂志占了39篇文章图.8



470b0004424364aace78

图.8 100篇文章杂志分析


我们再次统计了国家,发现美国有44篇,占了绝大部分,其次是英国,达到9篇;德国有6篇,很遗憾,没有发现有中国参与的文章图.9



470f000214e18ae35462

图.9 100篇文章国家分布


我们对研究机构统计了一下,发现加州大学系统占的比例最大,达到了7篇,其次是宾夕法尼亚州立大学,达到了4篇图.10


470a0004921d65a15306

图.10  100篇文章的大学或研究所分析



3引用量最高的100篇文章列表


对于这100篇文章,主要是技术的变革及基础研究,如蛋白质的定量,蛋白质的分析,实时定量PCR技术,BLAST的诞生,DNA测序,同源序列比对等,这些东西都是非常的基础,故这也注定了它们的高引用量图.11



标题总引用次数年均引用
1PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT3358445012.6
2CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF   BACTERIOPHAGE-T42447855099.69
3RAPID AND SENSITIVE METHOD FOR QUANTITATION OF MICROGRAM QUANTITIES OF   PROTEIN UTILIZING PRINCIPLE OF PROTEIN-DYE BINDING2013134793.17
4DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS667901629.02
5DENSITY-FUNCTIONAL THERMOCHEMISTRY .3. THE ROLE OF EXACT EXCHANGE652442609.76
6Generalized gradient approximation made simple634842885.64
7SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE   PHENOL CHLOROFORM EXTRACTION631612037.45
8DEVELOPMENT OF THE COLLE-SALVETTI CORRELATION-ENERGY FORMULA INTO A   FUNCTIONAL OF THE ELECTRON-DENSITY614062046.87
9A short history of SHELX586405864
10Analysis of relative gene expression data using real-time quantitative   PCR and the 2(T)(-Delta Delta C) method581593421.12
11ELECTROPHORETIC TRANSFER OF PROTEINS FROM POLYACRYLAMIDE GELS TO   NITROCELLULOSE SHEETS - PROCEDURE AND SOME APPLICATIONS551211413.36
12A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM   ANIMAL TISSUES51444843.34
13BASIC LOCAL ALIGNMENT SEARCH TOOL491161754.14
14CLUSTAL-W - IMPROVING THE SENSITIVITY OF PROGRESSIVE MULTIPLE SEQUENCE   ALIGNMENT THROUGH SEQUENCE WEIGHTING, POSITION-SPECIFIC GAP PENALTIES AND   WEIGHT MATRIX CHOICE474441976.83
15Gapped BLAST and PSI-BLAST: a new generation of protein database search   programs455572169.38
16NONPARAMETRIC-ESTIMATION FROM INCOMPLETE OBSERVATIONS44604743.4
17MINI-MENTAL STATE - PRACTICAL METHOD FOR GRADING COGNITIVE STATE OF   PATIENTS FOR CLINICIAN436371014.81
18A REVISED MEDIUM FOR RAPID GROWTH AND BIO ASSAYS WITH TOBACCO TISSUE   CULTURES41340738.21
19THE NEIGHBOR-JOINING METHOD - A NEW METHOD FOR RECONSTRUCTING   PHYLOGENETIC TREES387351249.52
20A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION37927642.83
21REVISED EFFECTIVE IONIC-RADII AND SYSTEMATIC STUDIES OF INTERATOMIC   DISTANCES IN HALIDES AND CHALCOGENIDES37143884.36
22DENSITY-FUNCTIONAL EXCHANGE-ENERGY APPROXIMATION WITH CORRECT   ASYMPTOTIC-BEHAVIOR340651135.5
23SELF-CONSISTENT EQUATIONS INCLUDING EXCHANGE AND CORRELATION EFFECTS33872639.09
24Efficient iterative schemes for ab initio total-energy calculations using   a plane-wave basis set336581529.91
25The moderator-mediator variable distinction in social psychological   research: conceptual, strategic, and statistical considerations.331911037.22
26Processing of X-ray diffraction data collected in oscillation mode326671555.57
27The status, quality, and expansion of the NIH full-length cDNA project:   The Mammalian Gene Collection (MGC)323912313.64
28DETECTION OF SPECIFIC SEQUENCES AMONG DNA FRAGMENTS SEPARATED BY   GEL-ELECTROPHORESIS32239749.74
29REGRESSION MODELS AND LIFE-TABLES32022696.13
30COLORIMETRIC METHOD FOR DETERMINATION OF SUGARS AND RELATED SUBSTANCES31882514.23
31RAPID COLORIMETRIC ASSAY FOR CELLULAR GROWTH AND SURVIVAL - APPLICATION   TO PROLIFERATION AND CYTO-TOXICITY ASSAYS31292894.06
32The CLUSTAL_X windows interface: flexible strategies for multiple   sequence alignment aided by quality analysis tools307701465.24
33HELICAL MICROTUBULES OF GRAPHITIC CARBON303861125.41
34STATISTICAL METHODS FOR ASSESSING AGREEMENT BETWEEN TWO METHODS OF   CLINICAL MEASUREMENT29847932.72
35FUZZY SETS29740561.13
36CONTROLLING THE FALSE DISCOVERY RATE - A PRACTICAL AND POWERFUL APPROACH   TO MULTIPLE TESTING296911290.91
37Electric field effect in atomically thin carbon films285202037.14
38INHOMOGENEOUS ELECTRON GAS27972518
39CONFIDENCE-LIMITS ON PHYLOGENIES - AN APPROACH USING THE BOOTSTRAP27721840.03
40SPECIAL POINTS FOR BRILLOUIN-ZONE INTEGRATIONS27045643.93
41MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood,   Evolutionary Distance, and Maximum Parsimony Methods265813797.29
42USE OF LEAD CITRATE AT HIGH PH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON   MICROSCOPY25405461.91
43From ultrasoft pseudopotentials to the projector augmented-wave method250571318.79
44MEASUREMENT OF OBSERVER AGREEMENT FOR CATEGORICAL DATA25005609.88
45PROJECTOR AUGMENTED-WAVE METHOD243141013.08
46MEGA4: Molecular evolutionary genetics analysis (MEGA) software version   4.0240662187.82
47RELIABILITY OF MOLECULAR WEIGHT DETERMINATIONS BY DODECYL   SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS23835486.43
48THE ATTRACTIONS OF PROTEINS FOR SMALL MOLECULES AND IONS23763344.39
49ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED   IN RIBONUCLEASE23574604.46
50The colorimetric determination of phosphorus23420251.83
51Cutoff Criteria for Fit Indexes in Covariance Structure Analysis:   Conventional Criteria Versus New Alternatives227551197.63
52Particle swarm optimization22586982
53DISC ELECTROPHORESIS .2. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS22463415.98
54MAXIMUM LIKELIHOOD FROM INCOMPLETE DATA VIA EM ALGORITHM22259542.9
55A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO   HIGH SPECIFIC ACTIVITY21557615.91
56Efficiency of ab-initio total energy calculations for metals and   semiconductors using a plane-wave basis set21539979.05
57NEW LOOK AT STATISTICAL-MODEL IDENTIFICATION21172481.18
58Global Cancer Statistics209832997.57
59A NEW GENERATION OF CA-2+ INDICATORS WITH GREATLY IMPROVED FLUORESCENCE   PROPERTIES20677626.58
60CLINICAL-DIAGNOSIS OF ALZHEIMERS-DISEASE - REPORT OF THE NINCDS-ADRDA   WORK GROUP UNDER THE AUSPICES OF DEPARTMENT-OF-HEALTH-AND-HUMAN-SERVICES   TASK-FORCE ON ALZHEIMERS-DISEASE20596605.76
61THE ASSESSMENT AND ANALYSIS OF HANDEDNESS: THE EDINBURGH INVENTORY20514436.47
62The rise of graphene205051864.09
63Distinctive image features from scale-invariant keypoints202581447
64A RATING SCALE FOR DEPRESSION20238348.93
65AN INVENTORY FOR MEASURING DEPRESSION20104352.7
66EQUATION OF STATE CALCULATIONS BY FAST COMPUTING MACHINES19396298.4
67ESTIMATION OF CONCENTRATION OF LOW-DENSITY LIPOPROTEIN CHOLESTEROL IN   PLASMA, WITHOUT USE OF PREPARATIVE ULTRACENTRIFUGE19380421.3
68HIGH-RESOLUTION 2-DIMENSIONAL ELECTROPHORESIS OF PROTEINS19179446.02
69COMPARISON OF SIMPLE POTENTIAL FUNCTIONS FOR SIMULATING LIQUID WATER19023543.51
70THE MOS 36-ITEM SHORT-FORM HEALTH SURVEY (SF-36) .1. CONCEPTUAL-FRAMEWORK   AND ITEM SELECTION18987730.27
71MicroRNAs: Genomics, biogenesis, mechanism, and function188451346.07
72PHASE ANNEALING IN SHELX-90 - DIRECT METHODS FOR LARGER STRUCTURES18787670.96
73A LOW-COST, HIGH-EFFICIENCY SOLAR-CELL BASED ON DYE-SENSITIZED COLLOIDAL   TIO2 FILMS18733693.81
74GAUSSIAN-BASIS SETS FOR USE IN CORRELATED MOLECULAR CALCULATIONS .1. THE   ATOMS BORON THROUGH NEON AND HYDROGEN18531639
75OPTIMIZATION BY SIMULATED ANNEALING18374524.97
76A NEW METHOD OF CLASSIFYING PROGNOSTIC CO-MORBIDITY IN   LONGITUDINAL-STUDIES - DEVELOPMENT AND VALIDATION18044582.06
77MrBayes 3: Bayesian phylogenetic inference under mixed models179941199.6
78TISSUE SULFHYDRYL GROUPS17989304.9
79VMD: Visual molecular dynamics17956816.18
80METAANALYSIS IN CLINICAL-TRIALS17945560.78
81IMPROVED PATCH-CLAMP TECHNIQUES FOR HIGH-RESOLUTION CURRENT RECORDING   FROM CELLS AND CELL-FREE MEMBRANE PATCHES17744479.57
82HOMEOSTASIS MODEL ASSESSMENT - INSULIN RESISTANCE AND BETA-CELL FUNCTION   FROM FASTING PLASMA-GLUCOSE AND INSULIN CONCENTRATIONS IN MAN17634534.36
83A SIMPLE METHOD FOR DISPLAYING THE HYDROPATHIC CHARACTER OF A PROTEIN17621489.47
84Measuring inconsistency in meta-analyses175841172.27
85MEASUREMENT OF PROTEIN USING BICINCHONINIC ACID17573532.52
86THE HOSPITAL ANXIETY AND DEPRESSION SCALE17458498.8
87STUDY OF THE CONDITIONS AND MECHANISM OF THE DIPHENYLAMINE REACTION FOR   THE COLORIMETRIC ESTIMATION OF DEOXYRIBONUCLEIC ACID17232277.94
88The Protein Data Bank17152952.89
89The NCEP/NCAR 40-year reanalysis project17118778.09
90Collective dynamics of 'small-world' networks17044852.2
91Hallmarks of Cancer: The Next Generation169132416.14
92ABINITIO MOLECULAR-DYNAMICS FOR LIQUID-METALS16788671.52
93Bias in meta-analysis detected by a simple, graphical test16785799.29
94DETERMINATION OF SERUM PROTEINS BY MEANS OF THE BIURET REACTION16754242.81
95A MATHEMATICAL THEORY OF COMMUNICATION16715238.79
96PROCHECK - A PROGRAM TO CHECK THE STEREOCHEMICAL QUALITY OF PROTEIN   STRUCTURES16710668.4
97MODELTEST: testing the model of DNA substitution16619830.95
98MULTIPLE RANGE AND MULTIPLE F TESTS16580263.17
99ESTIMATING DIMENSION OF A MODEL16551413.78
100A SIMPLE METHOD FOR ESTIMATING EVOLUTIONARY RATES OF BASE SUBSTITUTIONS   THROUGH COMPARATIVE STUDIES OF NUCLEOTIDE-SEQUENCES16492434

图.11  前100篇文章列表



注:所有的数据来源为Web of science。



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