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已有 3088 次阅读 2019-4-25 21:25 |系统分类:论文交流

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Golden bananas in the field: elevated fruit pro‐vitamin A from the expression of a single banana transgene

Vitamin A deficiency remains one of the world's major public health problems despite food fortification and supplements strategies. Biofortification of staple crops with enhanced levels of provitamin A (PVA) offers a sustainable alternative strategy to both food fortification and supplementation. As a proof of concept, PVAbiofortified transgenic Cavendish bananas were generated and field trialed in Australia with the aim of achieving a target level of 20 μg/g of dry weight (dw) βcarotene equivalent (βCE) in the fruit. Expression of a Fe'i bananaderived phytoene synthase 2a (MtPsy2a) gene resulted in the generation of lines with PVA levels exceeding the target level with one line reaching 55 μg/g dw βCE. 

Expression of the maize phytoene synthase 1 (ZmPsy1) gene, used to develop ‘Golden Rice 2’, also resulted in increased fruit PVA levels although many lines displayed undesirable phenotypes. Constitutive expression of either transgene with the maize polyubiquitin promoter increased PVA accumulation from the earliest stage of fruit development. In contrast, PVA accumulation was restricted to the late stages of fruit development when either the banana 1aminocyclopropane1carboxylate oxidase or the expansin 1 promoters were used to drive the same transgenes. Wildtype plants with the longest fruit development time had also the highest fruit PVA concentrations. The results from this study suggest that early activation of the ratelimiting enzyme in the carotenoid biosynthetic pathway and extended fruit maturation time are essential factors to achieve optimal PVA concentrations in banana fruit. 

(论文全文见https://onlinelibrary.wiley.com/doi/full/10.1111/pbi.12650




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