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Molecular Plant:拟南芥YABBY转录因子INO通过限制发育种子的铁积累,保证生殖成功

已有 2370 次阅读 2021-6-12 09:25 |个人分类:每日摘要|系统分类:论文交流

Restriction of Iron Loading into Developing Seeds by A YABBY Transcription Factor Safeguards Successful Reproduction in Arabidopsis

第一作者Li Sun

第一单位浙江大学

通讯作者Shao Jian Zheng


 Abstract 


背景回顾:Iron (Fe) storage in plant seeds is not only necessary for seedling establishment following germination, but also a major source of dietary Fe for human and animal life. 


提出问题Seed Fe accumulation was known to be low during early seed development. However, the underlying mechanism and biological significance remain elusive. 


结果1-INO过表达和降低表达表型:Here, we show that reduced expression of Arabidopsis YABBY transcription factor INNER NO OUTER (INO) increases embryonic Fe accumulation, while transgenic overexpression of INO has opposite effect.


结果2-INO表达模式及下游基因NRAMP1INO is highly expressed during early seed development, and that decreased expression of INO increases the expression of NATURAL RESISTANCE ASSOCIATED MACROPHAGE PROTEIN 1 (NRAMP1), a gene encoding a transporter contributing to Fe loading into the seed. 


结果3-NRAMP1作用于INO的下游-1:The relatively high embryonic Fe accumulation conferred by decreased expression of INO is rescued by the nramp1 loss-of-function mutation. 


结果4-INO结合NRAMP1启动子:We further demonstrate that INO represses NRAMP1 expression by binding to NRAMP1 specific promoter region. 


结果5-NRAMP1作用于INO的下游-2:Moreover, we show that excessive Fe loading into developing seeds in ino mutants produces more oxidative damage, leading to increased cell death and seed abortion, a phenotype that can be rescued by nramp1 mutation


结论:Taken together, these results indicate that INO plays an important role in safeguarding reproduction by reducing Fe loading into developing seeds via repression of NRAMP1 expression.


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 摘 要 


植物种子中的铁存储不仅是萌发后小苗生长所必需的,而且是人类和动物膳食铁的主要来源。在种子发育早期,种子铁积累保持在较低的水平。但是,潜在的分子机制以及生物学解释还不清楚。本文中,作者发现拟南芥YABBY转录因子INO表达量降低有助于胚胎铁积累的增加,而过表达INO具有相反的效果。INO基因在种子发育早期高表达,而INO基因的降低表达会增加一个作用于向种子运载铁的基因NRAMP1的高表达。nramp1功能缺失突变会使得INO表达量降低不在导致胚胎中高水平的铁积累。作者进一步的实验显示,INO可以通过直接结合到NRAMP1特异性启动子区域来抑制该基因的表达。此外,作者发现ino突变体正在发育的种子会积累过量的铁,引起更多的氧化应激损伤,导致细胞死亡增加、种子败育,而ino突变体的这种缺陷表型能够被nramp1突变拯救。综上,本文的研究结果揭示了INO通过抑制NRAMP1基因的表达,减少发育种子的铁积累,从而保证植物的生殖成功。


 通讯作者 

** 郑绍建 **


个人简介:

1986年,浙江农业大学,学士;

1991年,南京农业大学,博士。


研究方向主要从事植物响应铝毒、缺铁、重金属毒害等营养逆境的生理和分子机制的研究。


doi: https://doi.org/10.1016/j.molp.2021.06.005


Journal: Molecular Plant

Published date: June 07, 2021



https://blog.sciencenet.cn/blog-3158122-1290856.html

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