High‐Spatiotemporal‐Resolution Transcriptomes Provide Insights into Fruit Development and Ripening in Citrus sinensis

第一作者：Guizhi Feng

第一单位：华中农业大学

通讯作者：Hualin Yi

 Abstract 

背景回顾：Citrus fruit has a unique structure with soft leathery peel and pulp containing vascular bundles and several segments with many juice sacs. The function and morphology of each fruit tissue are different.

存在问题：Therefore, analysis at the organ‐wide or mixed‐ issue level inevitably obscures many tissue‐specific phenomena.

主要研究：High‐ hroughput RNA sequencing was used to profile Citrus sinensis fruit development based on four fruit tissue types and six development stages from young fruits to ripe fruits.

结果1-共表达网络：Using a coexpression network analysis, modules of coexpressed genes and hub genes of tissue‐specific networks were identified. Of particular importance is the discovery of the regulatory network of phytohormones during citrus fruit development and ripening.

结果2-ABA信号转导：A model was proposed to illustrate how ABF2 mediates the ABA signalling involved in sucrose transport, chlorophyll degradation, auxin homeostasis, carotenoid and ABA biosynthesis, and cell wall metabolism during citrus fruit development.

结果3-蔗糖和柠檬酸：Moreover, we depicted the detailed spatiotemporal expression patterns of the genes involved in sucrose and citric acid metabolism in citrus fruit and identified several key genes that may play crucial roles in sucrose and citric acid accumulation in the juice sac, such as SWEET15 and CsPH8.

结论：The high spatial and temporal resolution of our data provide important insights into the molecular networks underlying citrus fruit development and ripening.

 通讯作者 

**伊华林**

doi: https://doi.org/10.1111/pbi.13549

Journal: Plant Biotechnology Journal

First Published: Jan 20, 2021