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Plant Biotechnology Journal:高分辨率时空转录组揭示甜橙果实发育和成熟过程中的转录调控网络

已有 863 次阅读 2021-1-23 14:28 |个人分类:每日摘要|系统分类:论文交流

High‐Spatiotemporal‐Resolution Transcriptomes Provide Insights into Fruit Development and Ripening in Citrus sinensis

第一作者Guizhi Feng

第一单位华中农业大学

通讯作者Hualin Yi


 Abstract 


背景回顾Citrus fruit has a unique structure with soft leathery peel and pulp containing vascular bundles and several segments with many juice sacs. The function and morphology of each fruit tissue are different.


存在问题:Therefore, analysis at the organ‐wide or mixed‐ issue level inevitably obscures many tissue‐specific phenomena.


主要研究:High‐ hroughput RNA sequencing was used to profile Citrus sinensis fruit development based on four fruit tissue types and six development stages from young fruits to ripe fruits.


结果1-共表达网络:Using a coexpression network analysis, modules of coexpressed genes and hub genes of tissue‐specific networks were identified. Of particular importance is the discovery of the regulatory network of phytohormones during citrus fruit development and ripening.


结果2-ABA信号转导:A model was proposed to illustrate how ABF2 mediates the ABA signalling involved in sucrose transport, chlorophyll degradation, auxin homeostasis, carotenoid and ABA biosynthesis, and cell wall metabolism during citrus fruit development.


结果3-蔗糖和柠檬酸:Moreover, we depicted the detailed spatiotemporal expression patterns of the genes involved in sucrose and citric acid metabolism in citrus fruit and identified several key genes that may play crucial roles in sucrose and citric acid accumulation in the juice sac, such as SWEET15 and CsPH8.


结论:The high spatial and temporal resolution of our data provide important insights into the molecular networks underlying citrus fruit development and ripening.

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 Abstract 


柑橘果实具有独特的结构:柔软的革质果皮、由维管束组成的果肉以及一些含有汁囊的果瓣。而这每一个果实组织的功能和形态都是不同的。因此,在器官水平(果实)或者混样组织的分析,不可避免地掩盖了许多组织特异性的信息。本文中,作者通过高通量RNA测序对四种果实组织类型、六个发育时期(从幼果到成熟)的甜橙果实发育进行了转录图谱构建。利用共表达网络分析,作者鉴定了共表达基因模块以及组织特异性网络的核心基因。重中之重的是,作者构建了甜橙果实发育和成熟过程中激素相关的调控网络。作者构建了一个模型,用来解释甜橙果实发育过程中,ABF2如何介导ABA信号转导参与蔗糖转运、叶绿素降解、生长素内稳态、类胡萝卜素和ABA生物合成以及细胞壁代谢。此外,作者揭示了甜橙果实中参与蔗糖和柠檬酸代谢相关基因的时空表达模式,并且鉴定到了SWEET15CsPH8等可能参与汁囊中蔗糖和柠檬酸积累的关键基因。本文所报道的柑橘果实高分辨率时空转录组为柑橘果实发育和成熟过程中的分子网络提供了重要视野。


 通讯作者 

**伊华林**


个人简介:

1990年,华中农业大学,学士;

1996年,华中农业大学,硕士;

2004年,华中农业大学,博士。


研究方向:柑橘新品种选育和果实发育调控机理研究。

1. 常规杂交育种与芽变选种:通过常规杂交育种创制新种质和品种,对芽变种质资源进行鉴定和筛选获得优良品系; 

2. 柑橘果实发育与成熟调控分子机理解析:利用柑橘众多的熟期芽变材料和生物组学技术,挖掘调控柑橘果实成熟的重要路径和关键基因,以山金柑、番茄和草莓等为对象,通过遗传转化研究关键基因的功能,并分析其调控机制,继而采用转基因技术进行柑橘分子育种。


doi: https://doi.org/10.1111/pbi.13549


Journal: Plant Biotechnology Journal

First Published: Jan 20, 2021



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