Accessible chromatin regions and their functional interrelations with gene transcription and epigenetic modifications in sorghum genome

第一作者：Chao Zhou

第一单位：三峡大学

通讯作者：Chao Zhou

 Abstract 

背景回顾：Accessible chromatin regions (ACRs) provide physical scaffolds to recruit transcriptional co-regulators and displace their nearby nucleosomes in multiple plant species.

提出问题：Characterization of ACRs and investigation of their biological effects in Sorghum bicolor had been lagged behind.

研究基础：Regulation of gene expression relies on the transcriptional co-regulators that have been recruited to accessible chromatin regions (ACRs) to affect epigenomic modifications of surrounding nucleosomes.

主要研究：In the present study, we employed transposase-accessible chromatin sequencing (ATAC-seq) to identify ACRs and decipher how the presence of ACRs affects gene expression and epigenetic signatures in the Sorghum genome.

结果1-ACRs分布：As results, 21,077 ACRs which are mapped to 22.9% of genes and 2.7% of repeats were identified. The profiling of ACRs on gene structures reveals a narrow and sharp peak around the transcriptional start site (TSS), with relatively weak and broad signals covering the entire gene body and an explicit but wide peak beginning from the transcriptional termination site (TTS) to its downstream regions.

结果2-ACRs与基因表达：We discovered the correlations between gene expression levels and profiled ACR densities are dependent on the positions of ACRs. The occurrence of genic ACR (gACR) cumulatively enhances the transcriptional activity of intergenic ACR (iACR) associated genes.

结果3-ACRs与表观修饰：In addition, an intricate crosstalk among ACRs, gene expression, and epigenetic marks have been unveiled by integrating multiple-omics analyses of whole-genome bisulfite sequencing (BS-seq), 6mA immunoprecipitation followed by sequencing (6mA-IP-seq), RNA-seq, chromatin immunoprecipitation sequencing (ChIP-seq), and DNase I hypersensitive sites sequencing (DNase-seq).

结论：Our data provide a genome-wide landscape of ACRs in sorghum, decrypt their interrelations with various epigenetic marks, and shed light on their transcriptional regulation roles.

 摘 要 

 通讯作者 

**周超**

doi: https://doi.org/10.1016/j.xplc.2020.100140

Journal: Plant Communications

Published date: Dec 30, 2020