Flavonol‐mediated stabilization of PIN efflux complexes regulates polar auxin transport

第一作者：William D Teale

第一单位：弗莱堡大学

通讯作者：Klaus Palme

 Abstract 

背景介绍：The transport of auxin controls the rate, direction and localization of plant growth and development. The course of auxin transport is defined by the polar subcellular localization of the PIN proteins, a family of auxin efflux transporters.

提出问题：However, little is known about the composition and regulation of the PIN protein complex.

结果1-PIN蛋白复合体：Here, using blue‐native PAGE and quantitative mass spectrometry, we identify native PIN core transport units as homo‐ and heteromers assembled from PIN1, PIN2, PIN3, PIN4 and PIN7 subunits only.

结果2-黄酮醇：Furthermore, we show that endogenous flavonols stabilize PIN dimers to regulate auxin efflux in the same way as does the auxin transport inhibitor 1‐naphthylphthalamic acid (NPA).

结果3-抑制恢复：This inhibitory mechanism is counteracted both by the natural auxin indole‐3‐acetic acid and by phosphomimetic amino acids introduced into the PIN1 cytoplasmic domain.

结论：Our results lend mechanistic insights into an endogenous control mechanism which regulates PIN function and opens the way for a deeper understanding of the protein environment and regulation of the polar auxin transport complex.

doi: 10.15252/embj.2020104416

Journal: The EMBO Journal

Published date: Nov 13, 2020