TickingClock的个人博客分享 http://blog.sciencenet.cn/u/TickingClock

博文

EMBO J:拟南芥BIC1作为转录共激活子促进油菜素内酯信号转导和植物生长

已有 522 次阅读 2020-10-19 08:41 |个人分类:每日摘要|系统分类:论文交流

BIC1 acts as a transcriptional coactivator to promote brassinosteroid signaling and plant growth

第一作者Zongju Yang

第一单位中国农科院作物所

通讯作者Jiaqiang Sun


 Abstract 


背景+问题The BRASSINAZOLE‐RESISTANT 1 (BZR1) transcription factor family plays an essential role in plant brassinosteroid (BR) signaling, but the signaling mechanism through which BZR1 and its homologs cooperate with certain coactivators to facilitate transcription of target genes remains incompletely understood.


结果1-BZR1互作蛋白BIC1:In this study, we used an efficient protein interaction screening system to identify blue‐light inhibitor of cryptochromes 1 (BIC1) as a new BZR1‐interacting protein in Arabidopsis thaliana. We show that BIC1 positively regulates BR signaling and acts as a transcriptional coactivator for BZR1‐dependent activation of BR‐responsive genes.


结果2-BIC1与PIF4:Simultaneously, BIC1 interacts with the transcription factor PIF4 to synergistically and interdependently activate expression of downstream genes including PIF4 itself, and to promote plant growth.


结果3-免疫共沉淀:Chromatin immunoprecipitation assays demonstrate that BIC1 and BZR1/PIF4 interdependently associate with the promoters of common target genes.


结果4-演化保守性In addition, we show that the interaction between BIC1 and BZR1 is evolutionally conserved in the model monocot plant Triticum aestivum (bread wheat).


结论:Together, our results reveal mechanistic details of BR signaling mediated by a transcriptional activation module BIC1/BZR1/PIF4 and thus provide new insights into the molecular mechanisms underlying the integration of BR and light signaling in plants.


 摘  要 


BZR1转录因子家族在植物油菜素内酯(BR)信号转导过程中发挥重要作用,但是具体哪一个BZR1和其同源物与某个共激活子进行合作,促进靶基因的转录还不清楚。本研究中,作者利用一个高效的蛋白互作筛选系统,在拟南芥中鉴定到了一个新的BZR1互作蛋白,即隐花色素蓝光抑制子BIC1蛋白。作者发现BIC1能够正向调控BR信号转导,并且作为转录共激活子参与依赖于BZR1的BR响应基因的激活。同时,BIC1能够与转录因子PIF4互作,协调并且相互依赖地激活下游基因的表达,包括PIF4自身,以促进植物生长。染色质免疫共沉淀试验显示BIC1和BZR1/PIF4相互依赖,与共同靶基因的启动子区结合有关。另外,作者发现BIC1和BZR1之间的互作在单子叶模式植物面包小麦中也是保守的。综上,本文的结果揭示了由转录激活模块BIC1/BZR1/PIF4介导的BR信号转导机制,并且为植物中BR和光信号整合的分子机制提供了新的视野。


 通讯作者 


**孙加强**


个人简介:

1998年,山东农业大学,学士;

2001年,山东农业大学,硕士;

2005年,中科院遗传与发育生物学研究所,博士。


研究方向:小麦重要农艺性状的分子遗传学解析与分子设计育种。


doi: 10.15252/embj.2020104615


Journal: The EMBO Journal

Published date: Oct 14, 2020



http://blog.sciencenet.cn/blog-3158122-1254880.html

上一篇:PNAS:缺失免疫共受体BAK1会导致NLR依赖型的细胞死亡
下一篇:Genome Research:大片段串联重复影响基因表达、染色体3D结构以及植物-病原菌响应

0

该博文允许注册用户评论 请点击登录 评论 (0 个评论)

数据加载中...

Archiver|手机版|科学网 ( 京ICP备07017567号-12 )

GMT+8, 2020-11-27 00:06

Powered by ScienceNet.cn

Copyright © 2007- 中国科学报社

返回顶部