结果1-BZR1互作蛋白BIC1：In this study, we used an efficient protein interaction screening system to identify blue‐light inhibitor of cryptochromes 1 (BIC1) as a new BZR1‐interacting protein in Arabidopsis thaliana. We show that BIC1 positively regulates BR signaling and acts as a transcriptional coactivator for BZR1‐dependent activation of BR‐responsive genes.

结果2-BIC1与PIF4：Simultaneously, BIC1 interacts with the transcription factor PIF4 to synergistically and interdependently activate expression of downstream genes including PIF4 itself, and to promote plant growth.

结果3-免疫共沉淀：Chromatin immunoprecipitation assays demonstrate that BIC1 and BZR1/PIF4 interdependently associate with the promoters of common target genes.

结果4-演化保守性：In addition, we show that the interaction between BIC1 and BZR1 is evolutionally conserved in the model monocot plant Triticum aestivum (bread wheat).

结论：Together, our results reveal mechanistic details of BR signaling mediated by a transcriptional activation module BIC1/BZR1/PIF4 and thus provide new insights into the molecular mechanisms underlying the integration of BR and light signaling in plants.