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ISME J:羽纹硅藻响应于性诱导信息素的交配类型特异转录响应

已有 665 次阅读 2020-10-17 16:03 |个人分类:每日摘要|系统分类:论文交流

Mating type specific transcriptomic response to sex inducing pheromone in the pennate diatom Seminavis robusta

第一作者Gust Bilcke

第一单位根特大学

通讯作者Wim Vyverman


 Abstract 


背景回顾Sexual reproduction is a fundamental phase in the life cycle of most diatoms.


提出问题:Despite its role as a source of genetic variation, it is rarely reported in natural circumstances and its molecular foundations remain largely unknown.


主要研究:Here, we integrate independent transcriptomic datasets to prioritize genes responding to sex inducing pheromones (SIPs) in the pennate diatom Seminavis robusta.


结果1:We observe marked gene expression changes associated with SIP treatment in both mating types, including an inhibition of S phase progression, chloroplast division, mitosis, and cell wall formation. Meanwhile, meiotic genes are upregulated in response to SIP, including a sexually induced diatom specific cyclin.


结果2Our data further suggest an important role for reactive oxygen species, energy metabolism, and cGMP signaling during the early stages of sexual reproduction.


结果3-交配类型特异的SIP响应相关基因:In addition, we identify several genes with a mating type specific response to SIP, and link their expression pattern with physiological specialization, such as the production of the attraction pheromone diproline in mating type − (MT−) and mate-searching behavior in mating type + (MT+).


结论:Combined, our results provide a model for early sexual reproduction in pennate diatoms and significantly expand the suite of target genes to detect sexual reproduction events in natural diatom populations.


 摘  要 


有性生殖是大多数硅藻生命周期的一个重要阶段。尽管其是遗传变异其中之一的来源,但很少有报道揭示硅藻在自然环境下的有性生殖,并且其分子机制还不清楚。本文中,作者研究了独立的转录组数据,以筛选羽纹硅藻(Seminavis robusta)对性诱导信息素(sex inducing pheromones,简称SIPs)的响应基因。作者观察了与硅藻两种交配类型SIP处理后相关的标记基因表达变化,包括S期抑制、叶绿体分裂、有丝分裂以及细胞壁形成。同时,减数分裂基因在响应SIP时会上调表达,包括一个性诱导的硅藻特异细胞周期蛋白。作者的数据还显示,活性氧物质、能量代谢以及cGMP信号转导在有性生殖早期阶段发挥了重要的作用。另外,作者鉴定了一些基因存在交配类型特异性的SIP响应,并且将其表达与生理专化相互关联,比如交配类型MT−中会产生引诱信息素双脯氨酸,而交配类型MT+会存在伴侣搜索行为。综上,本文的结果为羽纹硅藻的早期有性生殖提供了一个模型,并且极大的拓宽了在自然群体中测试硅藻有性生殖事件的靶基因集。


 通讯作者 


**Wim Vyverman**


个人简介:

1995年,加州大学伯克利分校,博士。


研究方向:微生物的生物多样性、水生系统的生物地理环境历史、硅藻细胞与生命周期调控、化学通讯、物种形成、细胞大小感应、藻类生物技术等


doi: 10.1038/s41396-020-00797-7


Journal: The ISME Journal

Published date: Oct 07, 2020



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