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Current Biology:蚜虫的一种唾液蛋白激活植物的韧皮部防御

已有 907 次阅读 2020-10-15 09:02 |个人分类:每日摘要|系统分类:论文交流

An Aphid-Secreted Salivary Protease Activates Plant Defense in Phloem

第一作者Huijuan Guo

第一单位中科院动物研究所

通讯作者Yucheng Sun


 Abstract 


背景回顾Recent studies have reported that aphids facilitate their colonization of host plants by secreting salivary proteins into host tissues during their initial probing and feeding.


提出问题:Some of these salivary proteins elicit plant defenses, but the molecular and biochemical mechanisms that underlie the activation of phloem-localized resistance remain poorly understood.


研究对象:The aphid Myzus persicae, which is a generalized phloem-sucking pest, encompasses a number of lineages that are associated with and adapted to specific host plant species.


结果1-CathB3蛋白:The current study found that a cysteine protease Cathepsin B3 (CathB3), and the associated gene CathB3was upregulated in the salivary glands and saliva of aphids from a non-tobacco-adapted (NTA) aphid lineage, when compared to those of a tobacco-adapted lineage.


结果2-功能验证:Furthermore, the knockdown of CathB3 improved the performance of NTA lineages on tobacco, and the propeptide domain of CathB3 was found to bind to tobacco cytoplasmic kinase ENHANCED DISEASE RESISTANCE 1-like (EDR1-like), which triggers the accumulation of reactive oxygen species in tobacco phloem, thereby suppressing both phloem feeding and colonization by NTA lineages.


结论:These findings reveal a novel function for a cathepsin-type protease in aphid saliva that elicits effective host plant defenses and warranted the theory of host specialization for generalist aphids.


 摘  要 


最近的研究报道,蚜虫在刚开始刺吸和取食时,会将唾液蛋白分泌到宿主组织中,促进其在宿主植物中的定殖。这些唾液蛋白中的某些蛋白会诱导植物的防御,但是,目前我们对于韧皮部抗性激活的分子和生化机制仍然知之甚少。桃蚜(Myzus persicae)是一种通常吸食韧皮部的害虫,包含许多适应特定寄主植物的谱系。当前的研究发现,与适应烟草的蚜虫相比,半胱氨酸蛋白酶CathB3和相关基因CathB3在非烟草适应性蚜虫谱系的唾液腺和唾液中上调。此外,CathB3基因的敲低能够增强非烟草适应性蚜虫在在烟草上的表现,并且发现CathB3的前导肽结构域能够与烟草胞质激酶EDR1-like结合,从而诱导了烟草韧皮部活性氧的积累,因而抑制了非烟草适应性蚜虫对于烟草的取食和定殖。这些发现揭示了在蚜虫唾液中cathepsin蛋白酶具有诱导宿主植物有效防御的新功能,并为普通蚜虫的宿主专化提供了理论基础。


 通讯作者 


**孙玉诚**


个人简介:

1999-2003年,南昌大学,学士;

2004-2009年,中科院动物所,博士;

2011-2012年,美国Texas A&M University,博后。


研究方向:

(1)昆虫及其寄主植物对大气CO2浓度升高、臭氧增加、氮沉降、干旱等气候变化关键环境因子的响应机制;

(2)蚜虫-寄主植物-植物病毒互作中昆虫唾液腺组分的作用和功能。


doi: 10.1016/j.cub.2020.09.020


Journal: Current Biology

Published date: Oct 08, 2020



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