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Plant Communications:三七基因组

已有 960 次阅读 2020-9-24 19:29 |个人分类:每日摘要|系统分类:论文交流

The Chromosome-level Reference genome Assembly for Panax notoginseng and Insights into Ginsenoside Biosynthesis

第一作者Zhouqian Jiang

第一单位首都医科大学

通讯作者Wei Gao


 Abstract 


背景回顾Panax notoginseng, a perennial herb of the genus Panax in the family Araliaceae, has played an important role in clinical treatment in China for nearly thousands of years because of its extensive pharmacological effects.


主要研究:Here, we report a high-quality reference genome of P. notoginseng, whose genome size up to 2.66 Gb with a contig N50 of 1.12 Mb using third-generation PacBio sequencing technology, and which is the first chromosome-level genome of genus Panax.


结果1-WGD与皂苷合成:Through genome evolution analysis, we explored the phylogenetic and whole genome duplication (WGD) events, and also their impact on the biosynthesis of saponins.


结果2-块茎We further performed detailed transcriptional analysis of P. notoginseng and explored the gene-level regulatory mechanism of the formation of characteristic phenotype-tubercles.


结果3-皂苷合成:Then we studied the biosynthesis and regulation of saponins in temporal and spatial level, respectively.


结果4-萜类合成:Combining multi-omics data, we identified key enzyme genes involved in the terpenoid biosynthetic pathway of P. notoginseng.


结果5-人参皂苷:Finally, we identified 5 glycosyltransferase genes that could catalyze the formation of different ginsenosides in P. notoginseng.


结论:The genetic information obtained in this study provides a resource for further exploring the growth characteristics, cultivation, breeding and biosynthesis of saponins in P. notoginseng.


 摘  要 


三七是五加科人参属的多年生草本植物,因其广泛的药理学作用,在中国近千年的临床治疗中发挥了极其重要的作用。本文中,作者报道了第一个染色体水平的高质量三七参考基因组,通过三代PacBio测序技术,作者组装出的三七基因组大小为2.66Gb,contig N50的大小为1.12Mb。通过基因组演化分析,作者鉴定了系统发育与全基因组复制事件对于皂苷合成的影响。作者进一步对三七进行了详细的转录水平分析,揭示了三七特有的小块茎形成的基因调控机制。接着,作者还分别从时间和空间水平上,进一步研究了皂苷的合成和调控。结合多组学数据,作者鉴定了三七中参与萜类生物合成的关键酶。最终,作者鉴定到了5个糖基转移酶基因,在三七中催化不同的人参皂苷的形成。本文的所报道的三七全基因组数据为将来进一步研究三七生长特性、栽培、育种以及皂苷合成提供了遗传数据资源。


 通讯作者 


**高伟**


个人简介:

2005-2008年,中医科学院中药研究所,博士。


研究方向:

中药活性成分及天然药物基因功能鉴定、生源途径解析及合成生物学研究。


doi: 10.1016/j.xplc.2020.100113


Journal: Plant Communications

Published date: September 20, 2020



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