研究基础：Rather than succumbing to photodamage under extreme irradiance, unique features of photosystem II function allow C. ohadii to maintainhigh rates of photosynthesis and growth, accompanied by major changes in composition and cellular structure. This remarkable resilience allowed us to investigate the systems response of photosynthesis and growth to extreme illumination in a metabolically active cell.

主要研究：Using redox proteomics, transcriptomics, metabolomics and lipidomics, we explored the cellular mechanisms that promote dissipation of excess redox energy, protein S-glutathionylation, inorganic carbon concentration, lipid and starch accumulation, and thylakoid stacking.

结果：C. ohadii possesses a readily available capacity to utilize a sudden excess of reducing power and carbon for growth and reserve formation, and post-translational redox regulation plays a pivotal role in this rapid response. Frequently the response in C. ohadii deviated from that of model species, reflecting its life history in desert sand crusts. Comparative global and case-specific analyses provided insights into the potential evolutionary role of effective reductant utilization in this extreme resistance of C. ohadii to extreme irradiation.

 摘  要 

在两倍全日照的辐射下，小球藻Chlorella ohadii的表现远超我们的预期，说明目前我们对于植物光合作用机制如何运行、以及光合功能上限的了解还不够全面。比起在极端光辐射下由光损伤所导致的死亡，独特的光系统II功能特性使得小球藻能够维持高速率的光合和生长，并且伴随着组成和细胞结构的重大改变。小球藻的这种特性使得作者能够在代谢活跃的细胞中研究极端光照下的光合和生长响应。通过氧化还原蛋白质组学、转录组学、代谢组学以及脂质组学，作者研究了能够促进消耗过量的氧化还原能量、蛋白质S-谷胱甘肽化、无机碳浓度、脂质和淀粉积累以及类囊体堆积的细胞机制。小球藻具有一种随时待命的功能，可以利用突然产生的过量还原力和碳来进行生长和物质储备，而翻译后氧化还原调节在这种快速反应过程中起着至关重要的作用。小球藻的响应会经常偏离基于模型物种的预期，反映了其在沙漠沙土结皮中的生活史。通过对于整体和个例特异性的比较分析，作者揭示了有效的还原剂利用有助于小球藻在极端光照下所演化出的极端抗性。