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Plant Cell:拟南芥由类受体蛋白激酶CRK2介导的NADPH氧化酶C端磷酸化调控ROS产生

已有 502 次阅读 2020-6-27 08:41 |个人分类:每日摘要|系统分类:论文交流

CRK2 and C-terminal Phosphorylation of NADPH Oxidase RBOHD Regulate Reactive Oxygen Species Production in Arabidopsis

第一作者Sachie Kimura

第一单位赫尔辛基大学

通讯作者Michael Wrzaczek


 Abstract 


背景回顾:Reactive oxygen species (ROS) are important messengers in eukaryotic organisms, and their production is tightly controlled. Active extracellular ROS production by NADPH oxidases in plants is triggered by receptor-like protein kinase-dependent signaling networks.


主要发现:Here, we show that CYSTEINE-RICH RLK2 (CRK2) kinase activity is required for plant growth and CRK2 exists in a preformed complex with the NADPH oxidase RESPIRATORY BURST OXIDASE HOMOLOG D (RBOHD) in Arabidopsis (Arabidopsis thaliana).


主要发现:Functional CRK2 is required for the full elicitor-induced ROS burst, and consequently the crk2 mutant is impaired in defense against the bacterial pathogen Pseudomonas syringae pv tomato DC3000. Our work demonstrates that CRK2 regulates plant innate immunity. We identified in vitro CRK2-dependent phosphorylation sites in the C-terminal region of RBOHD. Phosphorylation of S703 RBOHD is enhanced upon flg22 treatment, and substitution of S703 with Ala reduced ROS production in Arabidopsis. Phylogenetic analysis suggests that phospho-sites in the C-terminal region of RBOHD are conserved throughout the plant lineage and between animals and plants.


结论:We propose that regulation of NADPH oxidase activity by phosphorylation of the C-terminal region might be an ancient mechanism and that CRK2 is an important element in regulating microbe-associated molecular pattern-triggered ROS production.

Schematic Model for MAMP-Triggered RBOHD Activation.


 摘  要 


真核生物中,活性氧物质ROS是重要的信号传递物质,其在生物体内的生产受到严格的调控。在植物中,由NADPH氧化酶介导的胞外ROS生产是由依赖于类受体蛋白激酶的信号网络所诱导的。本文中,作者发现富含半胱氨酸类受体蛋白CRK2激酶活性对于植物的生长是必需的,并且CRK2会与拟南芥中的NADPH氧化酶呼吸爆发氧化酶同源物RBOHD形成一个预先形成的复合物。激发子诱导的ROS完全爆发需要功能性的CRK2蛋白,而crk2突变体在对于细菌性病原体丁香假单胞菌防御存在缺陷。作者发现CRK2能够调控植物的天然免疫。作者在体外试验发现RBOHD的C端区域存在CRK2依赖性的磷酸化位点。flg22处理拟南芥会导致RBOHD蛋白703号位点丝氨酸的磷酸化增强,而将该位点替换为丙氨酸则会降低ROS的产量。系统发育树显示RBOHD蛋白C端的磷酸化位点在植物中比较保守,并且在动物和植物之间也是保守的。作者提出通过C端区域的磷酸化来调控NADPH氧化酶活性可能是一个比较古老的机制,而CRK2是调控微生物相关分子模式诱导ROS产生的关键组分。


p.s. flg22,最常见的激发子,是鞭毛蛋白N端保守的一个含22个氨基酸的小肽。常用于植物免疫研究中,处理植物细胞。


 通讯作者 


**Michael Wrzaczek**


个人简介:

2001年,维也纳大学,硕士;

2004年,维也纳大学,博士。


研究方向:

植物类受体蛋白激酶的功能及演化。


doi: 10.1105/tpc.19.00525


Journal: Plant Cell

Published date: February 07, 2020


p.s. 往期Michael Wrzaczek通讯研究链接:



http://blog.sciencenet.cn/blog-3158122-1239508.html

上一篇:Tree Physiology:银杏传粉滴代谢谱与miRNA研究
下一篇:Plant Cell:战姿预备~CRK2与RBOHD预结合并调节ROS生成(In brief)

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