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Tree Physiology:银杏传粉滴代谢谱与miRNA研究

已有 582 次阅读 2020-6-26 11:53 |个人分类:每日摘要|系统分类:论文交流

Liquid profiling in plants: identification and analysis of extracellular metabolites and miRNAs in pollination drops of Ginkgo biloba

第一作者Zhaogeng Lu

第一单位扬州大学

通讯作者Li Wang


 Abstract 


背景回顾:Pollination drop (PD), also known as an ovular secretion, is a critical feature of most wind-pollinated gymnosperms and functions as an essential component of pollination systems.


提出问题:However, the metabolome and small RNAs of gymnosperm PDs is largely unknown. 


结果-代谢:We employed gas chromatography–mass spectrometry to identify a total of 101 metabolites in Ginkgo biloba PDs. The most abundant metabolites were sugars (45.70%), followed by organic acids (15.94%), and alcohols (15.39%) involved in carbohydrate metabolism, glycine, serine and threonine metabolism. Through pollen culture by the PDs, we further demonstrated that the metabolic components of PDs are indispensable for pollen germination and growth, especially organic acids and fatty acids play defensive roles against microbial activity.


结果-miRNA:In addition, we successfully constructed a small RNA library and detected 45 known and 550 novel miRNAs in G. biloba PDs. Interestingly, in a comparative analysis of miRNA expression between PDs and ovules, we found that most of known miRNAs identified in PDs were also expressed in the ovules, implying that miRNAs in PDs may originate from ovules. Further, combining with potential target prediction, degradome validation and transcriptome sequencing, we identified that the interactions of several known miRNAs and their targets in PDs are involved in carbohydrate metabolism, hormone signaling, and defense response pathways, consistent with the metabolomics results.


结论:Our results broaden the knowledge of metabolite profiling and potential functional roles in gymnosperm PDs, and provide the first evidence of extracellular miRNA functions in ovular secretions from gymnosperms.


 摘  要 


传粉滴是一种胚珠分泌物,对于大多数以风媒传粉的裸子植物至关重要,是其授粉系统的重要一环。然而,裸子植物传粉滴的代谢组及小RNA相关研究仍然处于较大空白。本文中,作者通过气相色谱-质谱法鉴定了银杏传粉滴中101种代谢物。其中,含量最丰富的代谢物质为糖类,占了45.70%;其次是占了15.94%的有机酸以及15.39%的醇类,参与碳水化合物代谢,甘氨酸、丝氨酸和苏氨酸代谢。通过传粉滴的花粉培养,作者进一步发现传粉滴中的代谢组分对于花粉萌发和生长都是不可或缺的,尤其是其中的有机酸和脂肪酸起到了对于微生物活性的防御作用。另外,作者成功构建了一个小RNA文库,在银杏传粉滴中检测到了45种已知和550个新的miRNA。有趣的是,在传粉滴和胚珠的miRNA比较分析中,作者发现传粉滴中鉴定的已知miRNA大多在胚珠中也有表达,暗示传粉滴中的miRNA可能来源于胚珠。此外,结合miRNA的靶位点预测以及降解组验证和转录组测序,作者鉴定了传粉滴中一些已知miRNA与其靶基因之间的互作参与了碳水化合物代谢、激素信号和防御反应途径,与代谢组结果一致。本文的研究结果拓宽了目前对于裸子植物传粉滴代谢产物谱和潜在功能作用的认识,并首次证明了裸子植物中胚珠分泌中胞外miRNA的作用。


 通讯作者 


**王莉**


个人简介:

1993-1997年,扬州大学,学士;

2000-2003年,扬州大学,硕士;

2005-2008年,扬州大学,博士;

2010-2014年,中科院植物所,博士后。


研究方向:

银杏传粉滴与花粉成分检测及互作、银杏叶片活性成份合成调控。


doi: 10.1093/treephys/tpaa073


Journal: Tree Physiology

Published date: June 02, 2020



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