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Plant Biotechnology Journal:水稻GWAS鉴定了一个新的NLR基因作用于稻瘟病抗性

已有 861 次阅读 2019-11-21 16:44 |个人分类:每日摘要|系统分类:论文交流

Genome‐wide association study identifies an NLR gene that confers partial resistance to Magnaporthe oryzae in rice

First author: Ming‐Hao Liu; Affiliations: Institute of Plant Protection, Chinese Academy of Agricultural Sciences (中国农科院植物保护研究所): Beijing, China

Corresponding author: Guo‐Liang Wang

Because of the frequent breakdown of major resistance (R) genes, identification of new partial R genes against rice blast disease is an important goal of rice breeding. In this study, we used a core collection of the Rice Diversity Panel II (C‐RDP‐II), which contains 584 rice accessions and are genotyped with 700,000 single‐nucleotide polymorphism (SNP) markers. The C‐RDP‐II accessions were inoculated with three blast strains collected from different rice‐growing regions in China. Genome‐wide association study identified 27 loci associated with rice blast resistance (LABRs). Among them, 22 LABRs were not associated with any known blast R genes or QTLs. Interestingly, a nucleotide‐binding site leucine‐rich repeat (NLR) gene cluster exists in the LABR12 region on chromosome 4. One of the NLR genes is highly conserved in multiple partially resistant rice cultivars, and its expression is significantly up‐regulated at the early stages of rice blast infection. Knockout of this gene via CRISPR‐Cas9 in transgenic plants partially reduced blast resistance to four blast strains. The identification of this new non‐strain specific partial gene, tentatively named rice blast Partial Resistance gene 1 (PiPR1), provides genetic material that will be useful for understanding the partial resistance mechanism and for breeding durably resistant cultivars against blast disease of rice.


通讯:Guo-Liang Wang (https://plantpath.osu.edu/our-people/dr-guo-liang-wang)



doi: https://doi.org/10.1111/pbi.13300

Journal: Plant Biotechnology Journal

First Published: November 19, 2019



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