TickingClock的个人博客分享 http://blog.sciencenet.cn/u/TickingClock

博文

Nature Biotechnology:水稻抗细菌性枯萎病的诊断套件

已有 766 次阅读 2019-10-30 16:21 |个人分类:每日摘要|系统分类:论文交流

Diagnostic kit for rice blight resistance


First author: Joon-Seob Eom; Affiliations: Heinrich Heine University of Düsseldorf (杜塞尔多夫大学): Düsseldorf, Germany

Corresponding author: Wolf B. Frommer 


Blight-resistant rice lines are the most effective solution for bacterial blight, caused by Xanthomonas oryzae pv. oryzae (Xoo). Key resistance mechanisms involve SWEET genes as susceptibility factors. Bacterial transcription activator-like (TAL) effectors bind to effector-binding elements (EBEs) in SWEET gene promoters and induce SWEET genes. EBE variants that cannot be recognized by TAL effectors abrogate induction, causing resistance. Here we describe a diagnostic kit to enable analysis of bacterial blight in the field and identification of suitable resistant lines. Specifically, we include a SWEET promoter database, RT–PCR primers for detecting SWEET induction, engineered reporter rice lines to visualize SWEET protein accumulation and knock-out rice lines to identify virulence mechanisms in bacterial isolates. We also developed CRISPR–Cas9 genome-edited Kitaake rice to evaluate the efficacy of EBE mutations in resistance, software to predict the optimal resistance gene set for a specific geographic region, and two resistant ‘mega’ rice lines that will empower farmers to plant lines that are most likely to resist rice blight.




抗白叶枯病的水稻品系是解决由水稻黄单胞菌水稻致病变种Xoo引起的细菌性枯萎病最有效的方案。该过程最为核心的抗性机制涉及到SWEET基因作为易感性因子。细菌类转录激活因子TAL效应物会结合到SWEET基因启动子中的效应子结合元件EBE上,并诱导SWEET基因的表达。 某些水稻含有不能被TAL效应物识别的EBE变体,SWEET基因不会在致病菌侵染时诱导表达,从而获得抗性。本文中,作者报道了一种诊断试剂盒,可用于在大田环境下检测细菌性枯萎病,并鉴定合适的抗性系水稻。具体来说,该套工具包括了一个SWEET启动子数据库,用于检测SWEET诱导表达的RT-PCR引物,人工构建的水稻报告系以可视化SWEET蛋白的积累,以及水稻敲除系以鉴定细菌分离株中的毒力机制。作者还开发了CRISPR–Cas9基因组编辑的Kitaake水稻系,以评估EBE突变后对致病菌抗性的有效性,预测特定地理区域的最佳抗性基因集的软件,以及两个Mega水稻系,将有利于帮助农民种植最有可能抗枯萎病的水稻系。



通讯Wolf B. Frommer (http://www.molecular-physiology.hhu.de/en/our-team/curriculum-vitae.html)


研究方向:植物的营养成分转运过程,主要涉及作物正常生长状态下的产量以及受到病原物侵染时的产量。



doi: https://doi.org/10.1038/s41587-019-0268-y


Journal: Nature Biotechnology

Published date: October 28, 2019


085455gdrco4fe6dntl9e6.jpg



http://blog.sciencenet.cn/blog-3158122-1204088.html

上一篇:Nature Biotechnology:SWEET启动子编辑提升水稻对白叶枯病的广谱抗性
下一篇:New Phytologist:植物中COP1、HY5和BBXs介导的光信号转导研究综述

0

该博文允许注册用户评论 请点击登录 评论 (0 个评论)

数据加载中...

Archiver|手机版|科学网 ( 京ICP备14006957 )

GMT+8, 2020-1-23 16:43

Powered by ScienceNet.cn

Copyright © 2007- 中国科学报社

返回顶部