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Communications Biology:棉花对大丽轮枝菌抗性的分子调控机制

已有 473 次阅读 2019-6-23 16:37 |个人分类:每日摘要|系统分类:论文交流

Cotton plant defence against a fungal pathogen is enhanced by expanding BLADE-ON-PETIOLE1 expression beyond lateral-organ boundaries


First author: Zhennan Zhang; Affiliations: Institute of Microbiology, Chinese Academy of Sciences (中科院微生物研究所): Beijing, China

Corresponding author: Guixian Xia


In the plant response to pathogen infection, many genes’ expression is temporally induced, while few spatially induced expression genes have been reported. Here, we show that GhBOP1 can autonomously expand expression from restrained tissue when Gossypium hirsutum plants are attacked by Verticillium dahliae, which is considered to be spatially induced expression. Loss- and gain-of-function analyses show that GhBOP1 is a positive regulator in the modulation of plant resistance to V. dahliae. Yeast two-hybrid assays, luciferase complementation imaging and GUS reporting show that GhBOP1 interaction with GhTGA3 promotes its activation activity, regulating the expression of down-stream defence-related genes. Moreover, the induced spatial expression of GhBOP1 is accompanied by GhBP1 repression. Both antagonistically regulate the lignin biosynthesis, conferring cotton plants enhanced resistance to V. dahliae. Taken together, these results demonstrate that GhBOP1 is an economic positive regulator participating in plant defence through both the GhBOP1-GhTGA3 module and lignin accumulation.


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在植物响应于病原菌侵染的过程中,许多基因的表达会被暂时性的诱导,但目前只有少数几个在空间上诱导表达的基因被报道。本文中,作者的研究显示GhBOP1能够在陆地棉受到大丽轮枝菌侵染时,从其原先的表达区域自主地扩展出去,意味着该基因的表达受到了空间上的诱导。功能缺失和功能获得分析显示GhBOP1是棉花对于大丽轮枝菌抗性的正向调控因子。酵母双杂试验、荧光互补试验以及GUS报告系统显示GhBOP1能够与GhTGA3进行互作,促进其激活活性,从而调控下游抗性相关基因的表达。此外,GhBOP1空间诱导表达同时还伴随着GhBP1的抑制。这两者拮抗调控木质素的生物合成,赋予棉花植株增强版的大丽轮枝菌抗性。综上,本文的研究结果揭示了GhBOP1是一个通过GhBOP1-GhTGA3模块和木质素积累增强植物抗性的正向调控因子。



通讯:夏桂先 (http://www.im.cas.cn/jgsz2018/yjtx/zwjyzxgjzdsys/201811/t20181109_5168605.html)


个人简介:1973-1976年,武汉师范学院,学士;1989-1990年,瑞士日内瓦大学,硕士;1991-1993年,瑞士日内瓦大学,博士


研究方向棉花对黄萎病菌的抗病机制研究;棉花纤维发育的调控机制研究;马铃薯化肥农药减施增效技术的研发。



doi:  https://doi.org/10.1038/s42003-019-0468-5


Journal: Communications Biology

Published date: June 21, 2019


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http://blog.sciencenet.cn/blog-3158122-1186463.html

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