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Plant Physiology:生物钟基因TOC1作用于野生烟草干旱胁迫下的适应性

已有 501 次阅读 2019-6-11 19:46 |个人分类:每日摘要|系统分类:论文交流

The clock gene TOC1 in shoots, not roots, determines fitness of Nicotiana attenuata under drought


First author: Henrique A Valim; Affiliations: Max Planck Institute for Chemical Ecology (马克斯普朗克化学生态学研究所): Jena, Germany

Corresponding author: Ian T Baldwin


The highly conserved core circadian clock component TIMING OF CAB EXPRESSION 1 (TOC1) contextualizes environmental stress responses in plants, for example by gating abscisic acid (ABA) signaling and suppressing thermoresponsive growth. Selective interaction of TOC1 with PHYTOCHROME B (PHYB) under far-red-enriched light suggests a connection between circadian gating of light responses and sensitivity to ABA, an important regulator of growth and stress responses, including under drought. However, the fitness consequences of TOC1 function, particularly in the root, are poorly understood. Here, we used the desert annual, Nicotiana attenuata, to investigate the function of TOC1 in shoots and roots for maintaining fitness under drought, in both field and glasshouse experiments. Despite marked decreases in leaf water loss, TOC1-deficient (irTOC1) lines failed to maintain fitness in response to drought stress as measured by total seed capsule production. Restoring TOC1 transcript levels in shoots via micrografting was sufficient to restore wild-type drought responses under field conditions. Microarrays identified a co-expression module in leaves strongly linking red and far-red light signaling to drought responses in a TOC1-dependent manner, but experiments with phytochrome-deficient lines revealed that the effects of TOC1 deficiency under drought cannot be attributed to changes in red/far-red light perception alone. Taken together, these results elucidate the sophisticated, tissue-dependent role of the circadian clock in maintaining fitness in the face of long-term abiotic stresses such as drought.

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高度保守的核心生物钟组份TOC1协调植物的环境胁迫响应,比如控制脱落酸ABA信号转导和抑制温度响应的植物生长。TOC1与PHYB会选择性地在远红光富集的光照条件下发生互作,这说明植物光响应的生物钟控制和ABA敏感性之间存在某种关联,而植物对于ABA的敏感性是一个非常重要的生长和胁迫响应调控因素,包括在干旱条件下。然而,目前对于TOC1功能对于植物的适应性,尤其对植物根组织所带来的影响还不清楚。本文,作者利用荒漠一年生植物野生烟草在大田和温室环境中研究了TOC1在茎和根中作用于干旱适应性的功能。除了叶片水分丢失显著的降低,根据总体的种子蒴果产量测量结果显示TOC1功能缺陷突变体(irTOC1)株系不能很好地适应干旱胁迫。在大田条件下,通过微嫁接的技术恢复突变体中TOC1的转录水平,就足以恢复与野生型类似的干旱响应。芯片数据鉴定到了叶片中与红光和远红光信号通过一个TOC1依赖型的方式与干旱响应关联的共表达模块,但是进一步利用光敏色素缺陷突变体株系进行的试验结果显示TOC1功能缺陷在干旱胁迫下的影响并不足以单独改变植株对于红光/远红光的感知。综上,本文的研究结果揭示了生物钟作用于处于长期非生物胁迫环境条件下植物组织特异性的适应性机制。



通讯:Ian T. Baldwin (https://www.ice.mpg.de/ext/index.php?id=hopa&pers=iaba2016)


个人简介:1981年,达特茅斯学院,生物学学士;1989年,康奈尔大学,博士。


研究方向:生态系统中植物介导的相互作用。



doi: https://doi.org/10.1104/pp.19.00286


Journal: Plant Physiology

First Published: June 10, 2019




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