Nitric Oxide Deficiency Decreases CBF-dependent and CBF-independent Induction of Cold Acclimation

First author: álvaro Costa-Broseta; Affiliations: Universidad Politécnica de Valencia (瓦伦西亚理工大学): Valencia, Spain

Corresponding author: José León

Plant tolerance to freezing temperatures is governed by endogenous components and environmental factors. Exposure to low non-freezing temperatures is key factor in the induction of freezing tolerance in the process called cold acclimation. The role of nitric oxide (NO) in cold acclimation was explored in Arabidopsis thaliana using triple nia1,2 noa1-2 mutants that are impaired in the nitrate-dependent and nitrate-independent pathways of NO production, and are thus NO-deficient. The data presented here demonstrate that cold-induced NO accumulation is required to promote the full cold acclimation response through C-repeat Binding Factor (CBF)-dependent gene expression, as well as the CBF-independent expression of other cold-responsive genes such as the Oxidation-Related Zinc Finger 2 (ZF/OZF2). NO deficiency also altered abscisic acid perception and signaling and the cold-induced production of anthocyanins, which are additional factors involved in cold acclimation.

植物对于冰冻低温的耐受性是由内在遗传因素与外界环境因子所共同决定的。将植物暴露在非冻低温环境中生长一段时间是诱导植物获得冰冻低温抗性的关键手段，这一过程叫做植物的低温驯化。拟南芥的nia1,2 noa1-2三突变体在依赖于硝酸盐和独立于硝酸盐合成NO的通路都受损，所以该突变体是NO缺陷型突变体，通过该突变体可以研究NO在植物低温驯化中所发挥的作用。研究结果显示低温诱导的NO积累对于植物通过CBF依赖性基因表达以及ZF/OZF2等独立于CBF的其它低温响应基因的表达来促进植物完成完全的低温驯化是非常重要的。NO缺陷还能够改变植物对于ABA的感知和信号转导，以及低温诱导的花青素生成，这些是参与植物低温驯化的其它影响因子。

通讯：José León (http://www.ibmcp.csic.es/en/staff/jleonibmcpupves)

个人简介：1987年，塞维利亚大学，学士；1990年，塞维利亚大学，博士。

研究方向：植物胁迫与发育相互作用中的一氧化氮和植物激素。

doi: https://doi.org/10.1093/jxb/erz115

Journal: Journal of Experimental Botany

Published date: March 14, 2019