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Plant Biotechnol J:GT-1顺式元件参与水稻生物及非生物胁迫响应

已有 3222 次阅读 2017-10-17 08:30 |个人分类:每日摘要|系统分类:论文交流

OsASR2 regulates the expression of a defence-related gene, Os2H16, by targeting the GT-1 cis-element


First author:Ning Li; Affiliations: Shandong Agricultural University (山东农业大学): Shandong, China

Corresponding author: Zhaohui Chu (储昭辉)


The GT-1 cis-element widely exists in many plant gene promoters. However, the molecular mechanism that underlies the response of the GT-1 cis-element to abiotic and biotic stresses remains elusive in rice. We previously isolated a rice short-chain peptide-encoding gene (短链肽编码基因), Os2H16, and demonstrated that it plays important roles in both disease resistance and drought tolerance. Here, we conducted a promoter assay of Os2H16 and identified GT-1 as an important cis-element that mediates Os2H16 expression in response to pathogen attack and osmotic stress (渗透胁迫). Using the repeated GT-1 as bait, we characterized an abscisic acid, stress and ripening 2 (ASR2) protein from yeast-one hybridization screening. Sequence alignments showed that the carboxy-terminal domain (C末端结构域) of OsASR2 containing residues 80–138 was the DNA-binding domain. Furthermore, we identified that OsASR2 was specifically bound to GT-1 and activated the expression of the target gene Os2H16, as well as GFP driven by the chimeric promoter (嵌合启动子) of 2 × GT-1-35S mini construct. Additionally, the expression of OsASR2 was elevated by pathogens and osmotic stress challenges. Overexpression of OsASR2 enhanced the resistance against Xanthomonas oryzae pv. oryzae (水稻白叶枯病) and Rhizoctonia solani (立枯丝核菌), and tolerance to drought in rice. These results suggest that the interaction between OsASR2 and GT-1 plays an important role in the crosstalk of the response of rice to biotic and abiotic stresses.


GT-1顺式元件广泛存在于植物基因启动子上。然而GT-1顺式元件对于生物及非生物胁迫响应的潜在分子机制还有很多未解之谜。作者之前已经报道了一个水稻短链肽编码基因Os2H16参与水稻疾病抗性及干旱耐性。本文,作者进行了Os2H16的启动子试验,结果发现GT-1作为一个重要的顺式作用元件介导Os2H16在病原侵染及渗透胁迫响应的表达。利用重复的GT-1作为bait,通过酵母单杂试验作者鉴定了一个ASR2蛋白。序列比对显示OsASR2的C末端结构域存在一段80–138的残基是DNA结合结构域。此外,作者鉴定OsASR2特异性地结合到GT-1上,并激活其靶基因Os2H16的表达,正如由两个GT-1与35S一起驱动的GFP表达。另外,OsASR2的的表达在病原侵染和渗透胁迫后得到提升。过表达OsASR2基因会增强水稻对白叶枯病和立枯丝核菌的抗性,以及对干旱的耐性。以上的试验结果表明OsASR2和GT-1之间的互作在水稻应对生物及非生物胁迫的过程中起到重要作用。


通讯:储昭辉 (http://agronomy.sdau.edu.cn/s/37/t/1751/c8/6e/info51310.htm)



个人简介:博士、1977-,山东省植物病理学岗位泰山学者特聘教授、泰山学者海外特聘专家, 《植物病理学报》编委。1995-2006年华中农业大学完成本硕博连读,获得生物化学与分子生物学专业博士学位,2006-2008年英国The Sainsbury Laboratory博士后,2008-2014年山东农业大学植物保护学院教授。


研究方向:植物-微生物相互作用:1)细菌性病害与水稻的互作;2)真菌性病害与玉米和水稻的互作;3)马铃薯晚疫病与茄科的互作。


doi: 10.1111/pbi.12827


Journal: Plant Biotechnology Journal
First Published online: October 10, 2017.

P.S. 欢迎关注微信公众号:微信号Plant_Frontiers


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