文章：Gestational Diabetes is associated with changes in Placental Microbiota and Microbiome

杂志：Pediatric RESEARCH

时间：2016

链接：http://sci-hub.bz/10.1038/pr.2016.155

研究目的：We profiled the placental microbiota and microbiome in women with  gestational  diabetes (GDM) and studied its relation to maternal metabolism and placental expression of anti-inflammatory cytokines.

研究策略： The study population consists of 22 Caucasian women (11 with gestational diabetes and 11 control non-GDM women with normal pregnancies). PCR amplification was performed using 16S rRNA universal primers targeting the V3-V4 region of the bacterial 16S rRNA gene.

研究结果：Bacteria belonging to the Pseudomonadales order and Acinetobacter genus showed lower relative abundance in women with GDM compared to control (p<0.05).

个人想法：

      1）文章在数据分析上没有用太多力；

      2）课题设计上未来可以考虑，建立风险预测模型，如此在怀孕早期取样，结合肠道微生物、体重等维度的数据，实现早期风险预测；

      3）文中提到可考虑用益生菌去调节，针对高风险，可以考虑设置益生菌干预组去观察效果。