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13. Photoswitchable AIE-based H2S nanoprobe

已有 2406 次阅读 2018-6-3 10:42 |系统分类:论文交流

 

Photoswitchable AIE nanoprobe for lysosomal hydrogen sulfide detection and reversible dual-color imaging

Yongxiang Honga,bPeisheng Zhanga,*Hong WangaMaolin Yua,bYong Gaob,Jian Chena,* 


Current lysosomal H2S-responsive fluorescent probes usually possess notorious aggregation caused quenching (ACQ) phenomenon and suffer from the inevitable background fluorescence signals. To circumvent this problem, we here combined H2S recognizing aggregation-induced emission (AIE) group with photochromic spiropyran moiety to rational design novel photoswitchable AIE nanoprobe (DNBS-DCM-SP) for lysosomal H2S detection with high spatiotemporal resolution. In the presence of H2S, the electron-withdrawing DNBS moiety will deviate from DNBS-DCM-SP via a H2S-induced O-S bond cleavage, induced a sensitive turn-on fluorescence response at about 592 nm. The prepared nanoprobe displays good sensitivity (~5.0 nM), and high specificity (especial against other thiols). In additon, the fluorescence of the H2S-activated AIE nanoprobe (DCM-SP) can be reversibly switched by selective fluorescence resonance energy transfer (FRET) from the AIE fluorophore (DCM) to ring-opened state of spiropyran via alternating UV/visible light irradiation. Moreover, the feasibility of the prepared photoswitchable AIE nanoprobe is also demonstrated by mapping the production of endogenous H2S in living cells as well as reversible dual-color fluorescence imaging.


https://www.sciencedirect.com/science/article/pii/S0925400518310906


Sensors and Actuators B: Chemical, 2018,doi.org/10.1016/j.snb.2018.05.175



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