Recently, I need some RNA-seq experiments which have been published in NCBI for my study, while large sra file would take a lot of time in raw-data downloading (i.e. wget downloading). So I want to use aspera connect (command line version) to increse the rate for file downloading. The detail description was shown as follows:

1. Packages downloading

$ wget http://download.asperasoft.com/download/sw/connect/3.6.1/aspera-connect-3.6.1.110647-linux-64.tar.gz

2. Archive unzipping

$ tar -zxvf aspera-connect-3.6.1.110647-linux-64.tar.gz

3. Software installing

$ shaspera-connect-3.6.1.110647-linux-64.sh

4. Sra file downloading

5. Downloading command description

$ /path/to/ascp -i /path/to/openssh -k 1 -QT -l 200m /path/to/target/file /path/to/location

Notes: This command line operation was touchy to characters. For error reports avoiding, you should operate  as above description strictly. I attached my shell script which have been successfully run sra_down.txt.

Now I can download sra file fastly, while an error which was "Session Stop (Error: Session data transfer timeout (server), Peer Error: Session data transfer timeout)" always reproted. I can't find some available ways to solve it except tried repeatedly.